食品科学 ›› 2008, Vol. 29 ›› Issue (10): 254-256.

• 工艺技术 • 上一篇    下一篇

发酵液中金褐霉素的提取及其鉴定

 魏杰, 孟宪军, 李辉   

  1. 沈阳农业大学食品学院; 辽宁大学生命科学学院;
  • 出版日期:2008-10-15 发布日期:2011-12-08

Extraction and Identification of Aureofuscin from Fermentation Broth

 WEI  Jie, MENG  Xian-Jun, LI  Hui   

  1. 1.College of Food Science,Shenyang Agricultural University,Shenyang 110161,China; 2. Faculty of Life and Science,Liaoning University,Shenyang 110036,China
  • Online:2008-10-15 Published:2011-12-08

摘要: 采用大孔树脂吸附分离方法,从发酵液中提取金褐霉素并利用高效液相色谱对其进行定量检测。结果表明,发酵液采用大孔树脂吸附分离金褐霉素效果较好。紫外分光光度计扫描结果显示金褐霉素最大吸收波长为302.5nm。高效液相色谱归一化合分析表明样品中金褐霉素含量为98%。

关键词: 金褐霉素, 大孔吸附树脂, 紫外光谱, HPLC

Abstract: Macroporous resin was used to separate aureofuscin from fermentation broth of Streptomyces aureofuscus.n.sp,and hgih performance liquid chromatography (HPLC) method was adoted to determine aureofuscin quantitatively. The results showed that separation effect of aureofuscin is good by macroporous resin. The maximum absorption of aureofuscin is at 302.5 nm wavelength. The normalization analysis of HPLC indicated that aureofuscin content in the obtained sample by macroporous resin separation is 98%.

Key words: aureofuscin, macroporous resin, ultraviolet spectroscopy, HPLC