关键词: 分光光度法, 准噶尔铁线莲, 总黄酮, 含量测定

Abstract: In this study,the total flavonoids content was assayed by using spectrophotometry method with rutin as standard.The extraction technology of the total flavonoids from different parts of Clematis songarica Bunge,such as stem,leaf,flower and fruit was optimized by means of orthogonal test.Results showed that for the stem,the optimal extraction conditions are:ethanol concentration 50%,extraction temperature 50 ℃,extraction time 3 h and ratio of material to ethanol solution 1:50,and the absorbance of the total flavonoids extract is 0.270 under the optimal conditions.For the leaf,the optimal extraction condition are:ethanol concentration 60%,extraction temperature 70 ℃,extraction time 3 h and ratio of material to ethanol solution 1:30,and the absorbance of the total flavonoids extract is 0.562 under the optimal conditions.For the flower,the optimal extraction conditions are:ethanol concentration 50%,extraction temperature 60 ℃,extraction time 2 h and ratio of material to ethanol solution 1:50,and the absorbance of the total flavonoids extract is 0.480 under the optimal conditions.For the fruit,the optimal extraction conditions are:ethanol concentration 60%,extraction temperature 70 ℃,extraction time 3 h and ratio of material to ethanlo solution 1:40,and the absorbance of the total flavonoids extract is 0.365 under the optimal conditions.

Key words: spectrophotometry, Clematis songarica Bunge, total flavonoids, determination