食品科学 ›› 2007, Vol. 28 ›› Issue (2): 241-243.

• 分析检测 • 上一篇    下一篇

肉中微量氨的纳氏试剂光度法测定

 章丽燕, 王强, 余彬彬, 庄目德   

  1. 福建龙岩学院化学与材料工程系; 厦门大学化学化工学院化学系现代分析科学教育部重点实验室; 厦门大学化学化工学院化学系现代分析科学教育部重点实验室 福建龙岩364000 厦门大学化学化工学院化学系; 现代分析科学教育部重点实验室; 福建厦门361005;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Determination of Microamouts of Ammonia in Meat by Nessler’s Reagent Spectrophotometry

 ZHANG  Li-Yan, WANG  Qiang, YU  Bin-Bin, ZHUANG  Mu-De   

  1. 1.Department of Chemical and Material Engineering, Longyan College, Longyan 364000, China; 2.Key Laboratory of Analytical Sciences, Ministry of Education, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 本文提出了一种测定肉中粗氨的方法,可用于肉品新鲜度的检验。在常温下,纳氏试剂与肉中粗氨反应生成淡红棕色胶态化合物,最大吸收波长为400nm,表观摩尔吸光系数为1.404×104L/mol·cm,线性方程为y=0.52x+0.0001,r=0.9997,线性范围为0~1.0μg/ml,回收率在97.8%~104.8%之间。

关键词: 纳氏试剂, 粗氨, 肉, 光度法

Abstract: A method for the determination of the crude ammonia in meat was presented in this paper. The method based on the color reaction of ammonia-Nessler’s Reagent in room temperature. The results showed that the apparent molar absorptivity is 1.404 ×104 L/mol·cm at the wavelength of 400nm. Beer’s law is obeyed for the ammonia in the range of 0~1.0 μg/ml. The correlation coefficient is 0.9995. The recovery rate is 97.8%~104.8%. The method has been applied satisfactorily to crude ammonia in meat.

Key words: Nessler&rsquo, s reagent; crude ammonia; meat; spectrophotometry;