食品科学 ›› 2006, Vol. 27 ›› Issue (12): 208-213.

• 基础研究 • 上一篇    下一篇

鱼腥藻藻蓝蛋白的抗氧化作用

杨立红,王晓洁,钟旭升,冯培勇,李小青   

  1. 鲁东大学生命科学学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Detecting the Anti-oxidative Effect of Anabaena Phycocyanin

YANG Li-hong,WANG Xiao-jie,ZHONG Xu-sheng,FENG Pei-yong,LI Xiao-qing   

  1. College of Life Science, Ludong University, Yantai 264025, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 目的:探讨鱼腥藻藻蓝蛋白(AnabaenaPhycocyaninAPC)的体外抗氧化作用。方法:采用化学比色法测定APC在体外的(1)总抗氧化能力;(2)抑制活性超氧阴离子自由基(O2·)的能力;(3)清除羟自由基(·OH)的能力;(4)在其作用下的小鼠肝组织中脂质过氧化产物丙二醛(MDA)含量的变化。结果:一定浓度范围内不同纯度的APC均有总抗氧化能力,能清除O2·和·OH及抑制MDA的生成,且呈现一定剂量关系。结论:APC在一定浓度范围内具有抗氧化作用。

关键词: 鱼腥藻, 藻蓝蛋白, 抗氧化作用

Abstract: Aim: To detect the anti-oxidative effect of Anabaena Phycocyanin (APC) extracted from different media in vitro. Methods: Chemical colorimetry assay was used to test the anti-oxidative effect of APC in vitro including: ① The effect of totalanti-oxidation; ② The inhibiting effect on active superoxide anion (O·2 ); ③ The effect of eliminating hydroxyl radical (·OH); ④ The changes of MDA in liver of mice under the effect of APC. Results: APCs with different pour grades in some scope of concentrations all have the effects of the total anti-oxidation, the inhibiting active superoxide anion (O2·), the eliminating hydroxyl radical (·OH) and inhibiting the production of MDA in liver of mice, yet the effect was determined by dose of APC. Conclusion: APC has distinctively anti-oxidative effect in some scope of concentrations.

Key words:  , Anabaena; Phycocyanin; anti-oxidative effect;