金藻3011的培养条件优化及扩大培养研究

食品科学 ›› 2006, Vol. 27 ›› Issue (12): 253-259.

金藻3011的培养条件优化及扩大培养研究

王雪青，赵培，胡萍，彭丽丽，郝磊，程楚，刘玲玲，张蓉

天津商学院生物技术与食品科学学院天津市食品生物技术重点实验室；

出版日期:2006-12-15 发布日期:2011-11-23

Optimization of Cultural Conditions of Isochrysis galbana Parks 3011 and Its Mass-cultural

WANG Xue-qing,ZHAO Pei,HU Ping,PENG Li-li,HAO Lei,CHENG Chu, LIU Ling-ling,ZHANG Rong

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjing University of Commerce,Tianjing 300134, China

Online:2006-12-15 Published:2011-11-23

摘要/Abstract

摘要： 以金藻3011为材料,采用一次性培养方式,通过单因子梯度法研究了不同光照强度、温度、pH值对藻密度、细胞活性的影响;通过正交分析试验研究了N3+、P5+、Fe3+及NaCl添加量对金藻3011的生物量的影响,获得了以人工海水为基础的优化培养基配方;通过对比性试验研究了通气方式对自制6L光合生物反应器中高密度培养的金藻3011的生长速度和细胞密度的影响。结果表明:金藻3011适合在光照强度为3320lux,温度为24℃,偏碱性环境中生长,pH值为8.0,N3+、P5+、Fe3+浓度及NaCl添加量分别为0.04039g/L、0.0044g/L、3.90mg/L、20.476g/L时金藻3011生长最迅速,生物量及TTC细胞活力亦最高,生物量是优化前的1.432倍;以此培养条件及营养盐配比用于6L水平培养,在通气时间10min/次,通气次数3次/d的条件下,细胞干重可达到0.6613g/L,使最终生物量为优化前的1.815倍。

关键词: 金藻3011, 相对生长率, 营养盐, 培养条件, 优化

Abstract: With batch culture, the effect of temperature, light intensity and pH offer on the growth rate and maximual cell density of Isochrysis galbana Parks were studied in the work by gradient experiments. Nutrients including N3+, P5+, Fe3+, NaCl and the manner of CO2 were also studied by orthogonal or contrastive test. The results showed that light intensity 3220lux, temperature 24℃ are the optimum culture condition for Isochrysis galbana Parks. It liked growing in the alkaline environment, pH8.0. Concentrations of other nutrient factors were optimized by orthogonal test: N3+ 0.04039g/L, P5+ 0.0044g/L, Fe3+ 3.90mg/L and NaCl 20.476 g/L. Cultured with this optimal medium, the cell growth speed, TTC and biomass productivity increased significantly. The biomass was 1.432 times as much as before. The harvest yield (dry weight) was 0.6613g/L with the aeration rate 10min/ 3 time /day under the best cultural condition. The ultimate biomass reached the most, was 1.815 times as before especially.

Key words: Isochrysis galbana Parks 3011, growth rate, nutrients, cultural condition, optimization

王雪青，赵培，胡萍，彭丽丽，郝磊，程楚，刘玲玲，张蓉. 金藻3011的培养条件优化及扩大培养研究[J]. 食品科学, 2006, 27(12): 253-259.

WANG Xue-qing,ZHAO Pei,HU Ping,PENG Li-li,HAO Lei,CHENG Chu, LIU Ling-ling,ZHANG Rong. Optimization of Cultural Conditions of Isochrysis galbana Parks 3011 and Its Mass-cultural[J]. FOOD SCIENCE, 2006, 27(12): 253-259.

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