食品科学 ›› 2007, Vol. 28 ›› Issue (5): 190-194.

• 生物工程 • 上一篇    下一篇

Alcalase 2·4L FG酶解米渣蛋白动力学特性研究

 李积华, 郑为完, 苏冰霞, 张斌   

  1. 南昌大学食品科学教育部重点实验室; 南昌大学食品科学教育部重点实验室 江西南昌330047; 江西南昌330047;
  • 出版日期:2007-05-15 发布日期:2011-12-31

Study on Hydrolysis Kinetics Rice Dregs with Alcalase 2.4L FG

 LI  Ji-Hua, ZHENG  Wei-Wan, SU  Bing-Xia, ZHANG  Bin   

  1. Key Laboratory of Food Science,Ministry of Education,Nanchang University,Nanchang 330047,China
  • Online:2007-05-15 Published:2011-12-31

摘要: 采用pH-stat法对Alcalasc 2.4L FG酶水解米渣蛋白的动力学特性进行了比较系统探讨:(1)探讨了温度、酶浓度([E]/[S])、底物浓度、pH值和水解时间对水解度的影响,并确定了比较理想的工艺参数;(2)探讨了该酶催化水解米渣蛋白反应的动力学参数:Km=0.6598mol/L,Vmax=0.0035lmol/min·L;(3)探讨了该酶催化水解米渣蛋白反应的初级阶段的动力学特性,为米渣的进一步开发提供理论依据。

关键词: 米渣, 蛋白, 酶解, 动力学

Abstract: This paper discussed the hydrolysis kinetics on rice dregs with Alcalase 2.4L FG by pH-stat method:(1)The factors of reaction temperature,enzyme concentration,substrate concentration,pH value and reaction time were studied.(2) The maximum velocity and instant Km are determined by probing into the relation of the substrate concentration and reaction velocity:Km=0.6598mol/L,Vmax=0.00351mol/min·L.(3)Kinetics characteristics during the initial step of the enzymatic hydrolysis were discussed.

Key words: rice dregs, protein, enzymatic hydrolysis, kinetics