甜乳清粉中酪蛋白糖聚肽(CGMP)的分离及刺激小鼠肠道分泌IgA特性的研究

食品科学 ›› 2007, Vol. 28 ›› Issue (6): 327-331.

甜乳清粉中酪蛋白糖聚肽(CGMP)的分离及刺激小鼠肠道分泌IgA特性的研究

陈绪卓, 陈庆森

天津商业大学生物技术与食品科学学院生物工程系；天津300134 天津300134；天津300134天津市食品生物技术重点实验室；

出版日期:2007-06-15 发布日期:2011-12-31

Separation of Casein Glycomacropeptide(CGMP) from Whey Powder and Gut Mucosal SIgA Level by CGMP

CHEN Xu-Zhuo, CHEN Qing-Sen

1.Department of Bioengineering, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China;2.Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China

Online:2007-06-15 Published:2011-12-31

摘要/Abstract

摘要： 本实验将甜乳清粉中分离的酪蛋白糖聚肽(CGMP)用于研究对小鼠肠道分泌型IgA含量水平的影响,探讨CGMP对肠道免疫能力的调节作用。研究方法采用对健康小鼠灌喂CGMP制剂,分别在开始实验的2、5、7d实施小鼠解剖,刮取并收集肠黏膜液,最后进行肠道黏膜SIgA含量分析。结果表明,CGMP对小鼠肠道SIgA水平有比较明显的影响,表明对增强小鼠肠道黏膜免疫能力有重要作用。

关键词: 甜乳清粉, 酪蛋白糖聚肽, 黏膜免疫, SIgA

Abstract: The effect of CGMP on BABL/c gut mucosal secrete IgA level was investigated in this paper. The casein glycomacropeptide was separated from Crino whey powder. Gut mucosal immunomodulational ability by the stimulation of CGMP was explored. The method adopted was feeding BABL/c mice the preparation of CGMP, autopsying the experiment mice in 2, 5, 7d, and scraping and collecting mice gut mucous solution. The concentration of SIgA was determined by RIA method. The research results show that there was obvious influence of CGMP on the level of gut mucosal SIgA. Significant increase of gut mucosal immunomodulational ability was observed upon the stimulation of CGMP.

Key words: Crino whey powder, casein glycomacropeptide(CGMP), mucosal immune, secretory IgA(SIgA)

陈绪卓, 陈庆森. 甜乳清粉中酪蛋白糖聚肽(CGMP)的分离及刺激小鼠肠道分泌IgA特性的研究[J]. 食品科学, 2007, 28(6): 327-331.

CHEN Xu-Zhuo, CHEN Qing-Sen. Separation of Casein Glycomacropeptide(CGMP) from Whey Powder and Gut Mucosal SIgA Level by CGMP[J]. FOOD SCIENCE, 2007, 28(6): 327-331.

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