食品科学 ›› 2002, Vol. 23 ›› Issue (8): 217-221.
• 分析检测 • 上一篇 下一篇
刘光明, 苏文金, 陈向峰
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LIU Guang-Ming, SU Wen-Jin, CHEN Xiang-Feng
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摘要： 应用纯化的Bt1杀虫晶体蛋白作为标准蛋白和免疫抗原,通过抗体-抗原-酶标抗体反应,建立了酶联免疫吸附测定法(ELISA),以定量检测转基因玉米中的Bt1表达蛋白。用建立的ELISA法对4种进口玉米产品进行了测定,实验结果得到了免疫印迹分析的验证,并与进口试剂盒方法的定量分析结果相一致,因而建立的ELISA法具有操作简便、快速特异、定量准确、经济实惠的优点,特别适合于大批量检测,有着良好的应用前景。
关键词: 转基因玉米, Bt1蛋白, ELISA检测
Abstract: Based on the antibody-antigen-enzyme antibody reaction and by using puriftied Bt1 insecticidal crystal protein asboth standard protein and immunity antigen, a quantitative enzyme-linked immunosorbent assay (ELISA) method was estab-lished to detect express Bt1 protein in genetically modified maize.4 imported maize samples were determined by ELISA, Incomparison with the western-blotting & KIT assay method, the results were almost identical. It concluded that the ELISAmethod might be useful for easy,rapid,reliable and effective assay in large samples.
Key words: Genetically modified maize , Bt1 protein , ELISA detection
刘光明, 苏文金, 陈向峰. 应用ELISA定量检测转基因玉米中Bt1蛋白的研究[J]. 食品科学, 2002, 23(8): 217-221.
LIU Guang-Ming, SU Wen-Jin, CHEN Xiang-Feng. [J]. FOOD SCIENCE, 2002, 23(8): 217-221.
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