食品科学 ›› 2002, Vol. 23 ›› Issue (5): 31-33.

• 基础研究 • 上一篇    下一篇

野葛根异黄酮成分的超声萃取及抗氧化作用

 张尊听, 杨伯伦, 刘谦光, 薛东   

  1. 西安交通大学环境与化学工程学院,陕西师范大学化学系
  • 出版日期:2002-05-15 发布日期:2012-02-13

Study on Extracting Pueraia Isoflavonoid from Pueraria Lobata by the Method of Ultrasonic Wave and Its Antioxidative Effect

 ZHANG  Zun-Ting, YANG  Bo-Lun, LIU  Qian-Guang, XUE  Dong   

  • Online:2002-05-15 Published:2012-02-13

摘要: 采用超声法分别以95%乙醇、甲醇、70%乙醇和水为溶剂在不同时间水平从野葛根中萃取异黄酮活性成分,并用定性、定量的分析方法测定了野葛根提取物中异黄酮成分及其质量分数;同时,测定了在最佳条件下提取的葛根异黄酮对DDPH自由基的清除率。结果表明:以甲醇为溶剂超声萃取30分钟提取野葛根异黄酮成分效果最佳,提取率为20.6%,总黄酮质量分数为50.03%;采用超声萃取法从野葛根中提取异黄酮活性成分具有省时、节约能源、总黄酮提取率和产品纯度高的优点;葛根异黄酮具有较强的抗氧化作用,是一种天然的抗氧化剂。

关键词: 野葛, 葛根素, 异黄酮, 超声萃取, 抗氧化作用

Abstract: Pueraia isoflavonoid was extracted with ethanol, methanol, 70% ethanol and water in different time by the method of ultrasonic wave, respectively. The constituents and mass fraction of the Pueraia isoflavonoid were determined by qualitative and quantitative analysis. The results showed that the optimum technological conditions of extracting Pueraia isoflavonid from Pueraria Lobata by the method of ultrasonic wave were:the extracted with methanol was 30min, the extraction yield in the optimum conditions was 20.6% with mass fraction 50.03%. It was a saving time and energy, convenient and efficient way to extract Pueraia isoflavonoid from Puerarin; m P. Lobata. by the method of ultrasonic wave. Meanwhile, the Antioxidative Effect of Pueraia isoflavonoid in the optimum technological Conditions was determined by the method of DPPH. Pueraia isoflavonoid has a stronger antioxidative effect and is a natural antioxdiant.

Key words: Pueraria , Lobata(Willd.)Ohwi , Isoflavonoid , Ultrasonic wave extracting , Antioxidative effect