食品科学 ›› 2012, Vol. 33 ›› Issue (1): 98-102.doi: 10.7506/spkx1002-6630-201201019

• 基础研究 • 上一篇    下一篇

LTQ-Orbitrap 液- 质联用技术对水牛奶酪蛋白的鉴定

王丽娜,徐明芳*,成希飞,向明霞,李子超,李昀锴   

  1. 暨南大学生命科学技术学院,广东广州 510632
  • 收稿日期:2011-07-19 修回日期:2011-12-19 出版日期:2012-01-15 发布日期:2012-01-12
  • 通讯作者: 徐明芳 E-mail:xumingfang@jnu.edu.cn
  • 基金资助:

    广东省科技计划项目(2009B011300003)

Identification of Water Buffalo Caseins by Liquid Chromatography and LTQ-Orbitrap Mass Spectroscopy

WANG Li-na,XU Ming-fang*,CHENG Xi-fei,XIANG Ming-xia,LI Zi-chao,LI Yun-kai   

  1. College of Life Science and Technology, Jinan University, Guangzhou 510632, China
  • Received:2011-07-19 Revised:2011-12-19 Online:2012-01-15 Published:2012-01-12
  • Contact: XU Ming-Fang E-mail:xumingfang@jnu.edu.cn

摘要:

建立一种快速、高效的分析酪蛋白组分氨基酸序列的LTQ-Orbitrap质谱方法。为了研究水牛奶酪蛋白、乳牛奶酪蛋白和山羊奶酪蛋白的氨基酸序列组成及氨基酸的替换现象,采用LTQ-Orbitrap液-质联用技术分别对乳源酪蛋白的4种主要组分进行分析,搜索数据库获得4种组分的氨基酸全序列。与水牛奶4种酪蛋白组分进行比对。结果表明,乳牛奶的4种酪蛋白发生氨基酸替换的部位和比率明显小于山羊奶。这意味着与水牛奶酪蛋白相比,乳牛奶酪蛋白氨基酸的稳定性优于山羊奶酪蛋白。

关键词: 水牛奶, 酪蛋白, 氨基酸序列, LTQ-Orbitrap液-质联用技术

Abstract:

A rapid and efficient method to analyze the amino acid sequence of casein was presented using LTQ-Orbitrap mass spectroscopy coupled with liquid chromatography. In order to understand the amino acid sequence and substitution of cow, water buffalo and goat caseins, LTQ-Orbitrap mass spectroscopy coupled with liquid chromatography was used to analyze 4 major casein components, and their complete amino acid sequences were acquired by database searching. The amino acid substitutions of cow milk caseins were markedly lower than those of goat milk caseins. Thus, this study demonstrates that the amino acid sequences of cow milk caseins are more stable than those of goat milk caseins.

Key words: water buffalo milk, casein, amino acid sequence, casein structure, LTQ-Orbitrap mass spectrometry

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