响应面法优化油橄榄叶中橄榄苦苷超声提取工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (18): 73-76.

响应面法优化油橄榄叶中橄榄苦苷超声提取工艺

闫树军，王远，苏艳红，饶在生，李晖*

四川大学化学工程学院

收稿日期:2011-07-08 修回日期:2012-07-25 出版日期:2012-09-25 发布日期:2012-09-07
通讯作者: 李晖 E-mail:lihuilab@sina.com

Optimization of Ultrasound-Assisted Extraction Process for Oleuropein from Olea europaea Leaves by Response Surface Analysis

Received:2011-07-08 Revised:2012-07-25 Online:2012-09-25 Published:2012-09-07

摘要/Abstract

摘要： 目的：研究油橄榄叶中橄榄苦苷超声辅助提取最佳工艺条件。方法：选定甲醇体积分数、料液比和提取时间作为影响因素，以橄榄苦苷提取率为评价指标。在单因素试验的基础上，通过3因素3水平Box-Behnken试验，建立橄榄苦苷提取率的二次多项式回归方程，经响应面回归分析得到优化组合条件。结果：最佳工艺条件为甲醇体积分数93.5%、料液比1:25(g/mL)、提取时间26min。结论：油橄榄叶中橄榄苦苷提取率可达7.83%。

关键词: 油橄榄叶, 橄榄苦苷, 超声提取, 响应面分析

Abstract: Objective: To find optimal process conditions for the ultrasound-assisted extraction of oleuropein from Olea europaea leaves. Methods: The effects of methanol concentration, solid-to-solvent ratio and extraction time on oleuropein yield were investigated by one-factor-at-a-time design. A three-factor, three-level Box-Behnken experimental design was employed to build a second-order polynomial regression equation. The obtained regression equation was analyzed by response surface analysis to optimize the three process conditions. Results: The optimal process conditions were as follows: methanol concentration of 93.5%, solid-to-solvent ratio of 1:25 (g/mL) and an extraction time of 26 min. Conclusion: Under these conditions, the oleuropein yield from Olea europaea leaves was up to 7.83%.

Key words: Olea europaea leaves, oleuropein, ultrasound-assisted extraction, response surface methodology

中图分类号:

TS201.1

闫树军，王远，苏艳红，饶在生，李晖*. 响应面法优化油橄榄叶中橄榄苦苷超声提取工艺[J]. 食品科学, 2012, 33(18): 73-76.

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