食品科学 ›› 2012, Vol. 33 ›› Issue (18): 25-31.

• 工艺技术 • 上一篇    下一篇

黔江肾豆蛋白质提取工艺优化及其功能性质研究

熊家艳1,邓利玲1,范超敏1,邹泊羽1,钟 耕1,2,*     

  1. 1.西南大学食品科学学院 2.重庆市特色食品研究工程技术中心
  • 收稿日期:2011-07-27 修回日期:2012-08-01 出版日期:2012-09-25 发布日期:2012-09-07
  • 通讯作者: 钟耕 E-mail:zhongdg@126.com
  • 基金资助:

    重庆高校优秀成果转化项目

Optimization of Extraction Process and Functional Properties of Protein from Kidney Beans Grown in Qianjiang Region 

  • Received:2011-07-27 Revised:2012-08-01 Online:2012-09-25 Published:2012-09-07
  • Contact: Zhong Geng E-mail:zhongdg@126.com

摘要: 以黔江肾豆为原料,采用单因素法研究pH值、温度、液料比、提取时间等因素对其蛋白质提取率的影响,在此基础上通过响应面分析法优化得出最佳工艺条件碱提pH8.93、液料比10.67:1(mL/g)、提取时间22min、提取温度48℃,并对提取所得分离蛋白的持水力、吸油性、起泡性、乳化性等功能性质进行测定分析。

关键词: 黔江肾豆, 蛋白提取, 分离蛋白, 功能性质

Abstract: In this study, the effects of pH, temperature, material-to-liquid ratio, and extraction time on the extraction rate of   alkali-soluble protein (ASP) from kidney beans grown in Qiangjiang region were explored by one-factor-at-a-time design. The optimal process conditions for ASP extraction were determined by response surface methodology as pH 8.93, material-to-liquid ratio of 1:10.67, extraction time of 22 min, and extraction temperature of 48 ℃. The protein isolate obtained under these conditions was analyzed for functional properties such as water-holding capacity, oil-absorbing capacity, foaming properties and emulsifying properties.

Key words: kidney beans grown in Qiangjiang region, protein extraction, protein isolate, functional properties         

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