辣椒中β-隐黄质的分离制备

doi:10.7506/spkx1002-6630-201306003

食品科学 ›› 2013, Vol. 34 ›› Issue (6): 10-13.doi: 10.7506/spkx1002-6630-201306003

辣椒中β-隐黄质的分离制备

井凤1,2，王丽1,2，徐双双1,2，耿岩玲1，王明林2，王晓1,2,

1.山东省科学院分析测试中心，山东济南 250014；2.山东农业大学食品科学与工程学院，山东泰安 271018

收稿日期:2011-12-06 修回日期:2013-01-30 出版日期:2013-03-25 发布日期:2013-03-01
通讯作者: 王晓 E-mail:wangx@keylab.net
基金资助:
辣椒干制过程中红变机制及调控;中药药效物质标准化制备的技术体系研究;中药药效物质标准化制备的技术体系研究

Preparation of β-Cryptoxanthin from Paprika

JING Feng1,2，WANG Li1,2，XU Shuang-shuang1,2，GENG Yan-ling1，WANG Ming-lin2，WANG Xiao1,2,

1. Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, China；2. College of Food Science and Engineering, Shandong Agricultural University, Taian 271018 , China

Received:2011-12-06 Revised:2013-01-30 Online:2013-03-25 Published:2013-03-01

摘要/Abstract

摘要： 建立从辣椒中分离制备高纯度β-隐黄质的方法。辣椒粉先经丙酮超声提取、皂化，硅胶柱色谱进行粗分后进一步采用制备液相对β-隐黄质分离制备。通过紫外光、电喷雾电离质谱、核磁共振氢谱和核磁共振碳谱对所分离的β-隐黄质进行结构鉴定，分析型高效液相色谱进行纯度测定。结果表明：每5g辣椒色素浸膏与异丙醇的比例为1:9(g/mL)、加入6.5mL质量浓度为20g/100mL的KOH-甲醇溶液，在室温条件下皂化24h，皂化完全；硅胶柱色谱进行粗分时用石油醚与丙酮作为洗脱溶剂，当体积比为10:1时，β-隐黄质得到较好的富集；制备液相的条件为丙酮-水(95:5，V/V)、流速5mL/min，可制备分离出纯度为99.0%的β-隐黄质单体。该方法简便快捷、重现性好、所得β-隐黄质纯度高，可作为β-隐黄质的分离制备研究。

关键词: 辣椒, β-隐黄质, 制备高效液相色谱

Abstract: In order to establish a method for the separation and preparation of β-cryptoxanthin from paprika, the crude β-cryptoxanthin was extracted by ultrasonic acetone extraction, saponificated and separated by silica gel column chromatography. Preparative high performance liquid chromatography was used to refine and purify β-cryptoxanthin. The structure of β-cryptoxanthin was identified by UV, ESI-MS, 1H-NMR and 13C-NMR. The purity of β-cryptoxanthin was determined by analytical HPLC. The results indicated that complete saponification of chili pigment extract was achieved when saponifying its dispersion in 45 mL of isopropanol (9 g/mL) with 6.5 mL of 20% KOH methanol solution for 24 h at room temperature. During the rough separation by silica gel column chromatography using petroleum ether and acetone as elution solvents, β-cryptoxanthin could be better enriched at a volume ratio of 10:1. The preparative high performance liquid chromatography required acetone and water at a ratio of 95:5 (V/V) and a flow rate of 5 mL/min. Under these optimized conditions, β-cryptoxanthin with a purity of 99.0% could be obtained. This developed method is simple, rapid, reproductive, and suitable for the preparation of β-cryptoxanthin with high purity.

Key words: paprika, β-cryptoxanthin, preparative HPLC

中图分类号:

TS255.1

井凤1,2，王丽1,2，徐双双1,2，耿岩玲1，王明林2，王晓1,2,. 辣椒中β-隐黄质的分离制备[J]. 食品科学, 2013, 34(6): 10-13.

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辣椒中β-隐黄质的分离制备

Preparation of β-Cryptoxanthin from Paprika

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