响应面法优化枸杞叶粗多糖提取纯化工艺及其降血糖活性

食品科学 ›› 2013, Vol. 34 ›› Issue (4): 42-46.

响应面法优化枸杞叶粗多糖提取纯化工艺及其降血糖活性

江磊¹,梅丽娟²,刘增根¹,李洁琼²,王启兰¹,邵赟³,陶燕铎¹

1. 中国科学院西北高原生物研究所
2. 西北高原生物研究所
3. 中科院西北高原生物研究所

收稿日期:2011-12-08 修回日期:2013-01-10 出版日期:2013-02-25 发布日期:2013-01-29
通讯作者: 陶燕铎 E-mail:tyd@nwipb.ac.cn
基金资助:
科技部国家科技支撑计划

The research of the extracting, purification and the hypoglycemic effect of Lycium chinense. leaf polycose

Received:2011-12-08 Revised:2013-01-10 Online:2013-02-25 Published:2013-01-29

摘要/Abstract

摘要： 本文研究了西北地区特有的枸杞叶多糖的提取方法。作者采用响应面法对枸杞叶粗多糖提取中的料液比、提取温度和提取时间进行了优化，确定各因素最佳的水平组合。实验结果显示，在料液比1:47，提取温度72.9℃，提取时间2小时12分这个工艺条件下，枸杞叶粗多糖提取物中粗多糖的含量最高。然后作者采用D101大孔树脂和DEAE-52阴离子交换柱对目标活性多糖进行纯化，纯化后多糖含量为92.5%。接着用P-NPG法对目标多糖的糖苷酶抑制剂活性进行测定，目标多糖在1 mg/mL浓度下对5U/mL的糖苷酶抑制率达到93.7% 而阳性对照药阿卡波糖抑制率仅为21.7%。

Abstract: Response surface methodology (RSM) was applied to optimize the best extracting method of Lycium chinense. leaf. Model equation was proposed with regard to the effect of the ratio of Lycium chinense. leaf powder to water , temperature and time. The optimum conditions for the effect of the ratio of Lycium chinense. leaf powder to water , temperature and time obtained using RSM with regard to the content of polycose were 1:47, 72.9℃, and 2 h 12min respectively. The crude polycose was then purified by D 101 macroporous resin and DEAE-52 column. Phenol-sulfuric acid method was chosen to test the purity of sample which was up to 92.5%. The anti-diabetic activity of the polycose was assessed using enzymatic inhibitory assays against the α-glycosidase. The percentage of the inhibition of U/mL α-glycosidase is 93.7% however the percentage of the inhibition of control acarbose was only 21.7%.

中图分类号:

江磊梅丽娟刘增根李洁琼王启兰邵赟陶燕铎. 响应面法优化枸杞叶粗多糖提取纯化工艺及其降血糖活性[J]. 食品科学, 2013, 34(4): 42-46.

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