食品科学 ›› 2013, Vol. 34 ›› Issue (11): 138-141.doi: 10.7506/spkx1002-6630-201311031

• 基础研究 • 上一篇    下一篇

玫瑰花瓣总黄酮和总多糖的体外抗氧化活性

帕尔哈提·柔孜,阿依姑丽·艾合麦提*,朱 昆,刘 璐,胡 月,何文婷   

  1. 新疆农业大学食品科学与药学学院,新疆 乌鲁木齐 830052
  • 收稿日期:2012-04-09 修回日期:2013-05-07 出版日期:2013-06-15 发布日期:2013-06-03
  • 通讯作者: 阿依姑丽·艾合麦提 E-mail:glcpau@163.com

Antioxidant Activity in vitro of Total Flavonoids and Total Polysaccharides from Rose Petals

Parhat·ROZI,Aygul·AHMAT*,ZHU Kun,LIU Lu,HU Yue,HE Wen- ting   

  1. College of Food Science and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, China
  • Received:2012-04-09 Revised:2013-05-07 Online:2013-06-15 Published:2013-06-03

摘要:

为研究新疆和田玫瑰花瓣总黄酮和总多糖的体外抗氧化活性,以VC作为阳性对照,测定羟自由基(·OH)清除率、超氧阴离子自由基(O2ˉ·)清除率、DPPH自由基清除率和肝脏自氧化过程的抑制率。结果表明:玫瑰花瓣总黄酮、总多糖对·OH、O2ˉ·、DPPH自由基均有不同程度的清除作用。总黄酮在一定的质量浓度范围内对·OH、O2ˉ·和DPPH自由基的清除率呈良好线性关系,半数抑制率(IC50)分别为2.21、2.39、2.54μg/mL;总多糖在一定的质量浓度范围内对·OH、O2ˉ·和DPPH自由基的清除率呈良好线性关系,其IC50分别为2.78、2.86、3.44μg/mL;玫瑰花瓣总黄酮、总多糖对脂质过氧化亦有明显的抑制作用,总黄酮、总多糖最大抑制率分别为49.87%、65.52%,并呈现质量浓度依赖。玫瑰花瓣总黄酮清除·OH、O2ˉ·、DPPH自由基的能力均强于总多糖,而总多糖抑制脂质过氧化作用优于总黄酮。

关键词: 和田玫瑰, 总黄酮, 总多糖, 抗氧化活性

Abstract:

Using VC as a positive control, the antixoidant activities of total flavonoids and total polysaccharides from rose
petals collected from Hotan, Xinjiang were evaluated by scavenging rates against DPPH, superoxide anion and hydroxyl
radicals and inhibitory rate against lipid oxidation of liver homogenate. Both total flavonoids and total polysaccharides
from rose petals had scavenging effects on DPPH, superoxide anion and hydroxyl radicals. Over the studied concentration
range, the radical scavenging activity of both extracts showed linear concentration dependence. In DPPH, superoxide anion
and hydroxyl radical scavenging assays, the IC50 of the total flavonoid extract were 2.21, 2.39 μg/mL and 2.54 μg/mL,
respectively, compared to 2.78, 2.86 μg/mL and 3.44 μg/mL for the total polysaccharide extract. Moreover, both extracts
possessed potent inhibitory effects on lipid peroxidation in a dose-dependent manner, with maximum inhibory rates of
49.87% and 69.52%, respectively. The total flavonoid extract exhibted stronger fradical scavenging activity than the total
flavonoid extract despite having anti-lipid peroxidation activity.

Key words: Hotan grown rose, total flavonoids, polysaccharides, antioxidant activity