响应面优化超声辅助提取羊栖菜中无机砷

doi:10.7506/spkx1002-6630-201310015

食品科学 ›› 2013, Vol. 34 ›› Issue (10): 67-71.doi: 10.7506/spkx1002-6630-201310015

响应面优化超声辅助提取羊栖菜中无机砷

张金玲，钟耀广，万金庆，乔勇进

1.上海海洋大学食品学院，上海水产品加工及贮藏工程技术研究中心，上海 201306；
2.上海市农业科学院农产品保鲜加工研究中心，上海 201403

收稿日期:2012-04-11 修回日期:2013-03-27 出版日期:2013-05-25 发布日期:2013-05-07
通讯作者: 钟耀广 E-mail:ygzhong@shou.edu.cn
基金资助:
上海市教委食品质量与安全重点学科开放基金资助项目

Optimization of Ultrasonic-Assisted Extraction of Inorganic Arsenic in Sargassum fusiforme (Harv.) by Response Surface Methodology

ZHANG Jin-ling，ZHONG Yao-guang，WAN Jin-qing，QIAO Yong-jin

1. College of Food Science and Technology, Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai Ocean University, Shanghai 201306, China；2. Agricultural Products Storage and Processing Research Center, Shanghai Academy of Agriculture Science, Shanghai 201403, China

Received:2012-04-11 Revised:2013-03-27 Online:2013-05-25 Published:2013-05-07
Contact: Yao-Guang ZHONG E-mail:ygzhong@shou.edu.cn

摘要/Abstract

摘要：

采用盐酸作为提取剂，超声辅助提取法对羊栖菜中的无机砷进行提取，氢化物发生-原子荧光光谱法(HGAFS)测定羊栖菜中的无机砷。为了优化羊栖菜中无机砷的提取工艺，在单因素试验基础上，利用Box-Behnken试验设计原理和响应面分析法，以荧光强度为响应值，选取浸提液浓度、超声提取温度和提取时间3个因素，研究各因素及其交互作用对荧光强度的影响。结果显示：无机砷的最佳提取条件为浸提液浓度6.6mol/L、提取温度53.4℃、提取时间24.4min，在此条件下，检测所得荧光强度为675.51，通过计算得无机砷含量为4.78mg/kg。

关键词: 原子荧光法, 羊栖菜, 无机砷, 超声提取, 响应面

Abstract:

Ultrasonic-assisted extraction was utilized to extract inorganic arsenic from Sargassum fusiforme (Harv.)
by hydrochloric acid. Hydride generation atomic fluorescence spectrometry (HG-AFS) was used for the determination
of inorganic arsenic. Based on the single factor tests, the Box-Behnken central composite design and response surface
methodology (RSM) were adopted to obtain the optimal conditions for the extraction of inorganic arsenic from Sargassum
fusiforme (Harv.), using fluorescence intensity as the response value and the extraction concentration, extraction temperature
and time as the factors. The results showed that the optimum extraction conditions of inorganic arsenic were the extraction
concentration 6.6 mol/L, extraction temperature 53.4 ℃ and time 24.4 min. Under these conditions, the fluorescence
intensity was 675.51, and the content of inorganic arsenic was 4.78 mg/kg.

Key words: atomic fluorescence spectrometry (AFS), Sargassum fusiforme (Harv.), inorganic arsenic, ultrasonicassisted extraction, response surface methodology

中图分类号:

中图分类号：O613.63 文献标识码：A

张金玲，钟耀广，万金庆，乔勇进. 响应面优化超声辅助提取羊栖菜中无机砷[J]. 食品科学, 2013, 34(10): 67-71.

ZHANG Jin-ling，ZHONG Yao-guang，WAN Jin-qing，QIAO Yong-jin. Optimization of Ultrasonic-Assisted Extraction of Inorganic Arsenic in Sargassum fusiforme (Harv.) by Response Surface Methodology[J]. FOOD SCIENCE, 2013, 34(10): 67-71.

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响应面优化超声辅助提取羊栖菜中无机砷

Optimization of Ultrasonic-Assisted Extraction of Inorganic Arsenic in Sargassum fusiforme (Harv.) by Response Surface Methodology

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