关键词: 金银木, 色素, 超声提取, 响应面法

Abstract:

Following a period of spontaneous solvent extraction at a constant temperature, ultrasonication was applied to
enhance pigment extraction from crushed Lonicera maackii fruit. Three extraction parameters including material/solvent ratio,
ultrasonication time and power were optimized by using response surface methodology. All the operating parameters had
a significant effect on extraction efficiency and the optimal extraction conditions were determined as 1:15 (g/mL), 30 min
and 500 W for material/solvent ratio, ultrasonication time and power, respectively. The predicted absorbance at 450 nm of
the pigment extracted under the optimized conditions after 10-fold dilution was 0.836, showing a relative error of only 1.08%
in comparison to the experimental absorbance of 0.827. Thus the optimized process established using response surface methodology
is feasible for pigment extraction from Lonicera maackii fruit.

Key words: Lonicera maackii, pigment, ultrasonic wave extraction, response surface methodology