L-苹果酸对大鼠转运蛋白及抗氧化酶基因表达的影响

doi:10.7506/spkx1002-6630-201323056

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 276-281.doi: 10.7506/spkx1002-6630-201323056

L-苹果酸对大鼠转运蛋白及抗氧化酶基因表达的影响

曾晓琮1,2，吴军林2，肖春2，吴清平2，徐明芳1，张菊梅2,*

1.暨南大学生命科学技术学院，广东广州 510006；2.广东省微生物研究所，省部共建华南应用微生物国家重点实验室，
广东省菌种保藏与应用重点实验室，广东省微生物应用新技术公共实验室，广东广州 510070

收稿日期:2013-07-18 修回日期:2013-11-04 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 张菊梅 E-mail:Zhangjm926@126.com
基金资助:
国家自然科学基金项目(31000762)；广州市珠江科技新星专项(2013J220079)

Effect of L-malate on Gene Expression of Carrier Proteins and Antioxidant Enzymes in Rats

ZENG Xiao-cong1,2，WU Jun-lin2，XIAO Chun2，WU Qing-ping2，XU Ming-fang1，ZHANG Ju-mei2,*

1. College of Life Science and Technology, Jinan University, Guangzhou 510006, China；
2. State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Provincial
Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong
Institute of Microbiology, Guangzhou 510070, China

Received:2013-07-18 Revised:2013-11-04 Online:2013-12-15 Published:2014-01-03
Contact: ZHANG Ju-mei E-mail:Zhangjm926@126.com

摘要/Abstract

摘要：

对12月龄SD大鼠给予L-苹果酸30d后，采用含有SYBR Green I的Real Time RT-PCR法，对肝脏和心脏线粒体苹果酸天冬氨酸穿梭中的两种转运蛋白(天冬氨酸谷氨酸转运蛋白(AGC)与α-酮戊二酸苹果酸转运蛋白(OMC))以及两种抗氧化酶(过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px))的基因表达进行检测，以研究L-苹果酸增强线粒体抗氧化作用的分子生物学机制。结果表明：苹果酸组中大鼠心肌细胞AGC、OMC、CAT、GSH-Px的mRNA表达量分别是空白对照组的1.25、1.39、1.12、1.01倍。苹果酸组中大鼠肝脏细胞AGC、OMC、CAT、GSH-Px的mRNA表达量分别是空白对照组的1.33、1.02、1.25、0.94倍。由此推测，L-苹果酸可能通过促进苹果酸天冬氨酸穿梭蛋白以及抗氧化酶的基因表达，实现提高线粒体的抗氧化作用。

关键词: 线粒体抗氧化, 苹果酸-天冬氨酸穿梭, 实时反转录聚合酶链式反应

Abstract:

To investigate the antioxidant mechanism of L-malate on mitochondria, real time RT-PCR was used to analyze
the gene expressions of two carrier proteins (AGC and OMC) in the inner membrane and two antioxidant enzymes (CAT
and GSH-Px) related to the malate-aspartate shuttle in rat liver and heart mitochondria. The mRNA expressions of these
proteins were examined by real time RT-PCR in hearts and livers of SD rats treated with L-malate. The results indicated that
the mRNA expressions of AGC, OMC, CAT and GSH-Px in the heart of rats treated with L-malate were 1.25, 1.39, 1.12, 1.01
times as much as those in the control group. The mRNA expression of AGC, OMC, CAT and GSH-Px in the liver of rats
treated with L-malate was 1.33, 1.02, 1.25, 0.94 times as much as those in the control group. These results imply that L-malate
might increase the antioxidant capacity of mitochondria by enhancing the mRNA expression of the proteins involved in the
malate-aspartate shuttle and the antioxidant enzymes.

Key words: mitochondria antioxidant capacity, malate-aspartate shuttle, real-time reverse transcription polymerase chain reaction (real-time RT-PCR)

中图分类号:

Q812

曾晓琮1,2，吴军林2，肖春2，吴清平2，徐明芳1，张菊梅2,*. L-苹果酸对大鼠转运蛋白及抗氧化酶基因表达的影响[J]. 食品科学, 2013, 34(23): 276-281.

ZENG Xiao-cong1,2，WU Jun-lin2，XIAO Chun2，WU Qing-ping2，XU Ming-fang1，ZHANG Ju-mei2,*. Effect of L-malate on Gene Expression of Carrier Proteins and Antioxidant Enzymes in Rats[J]. FOOD SCIENCE, 2013, 34(23): 276-281.

参考文献

[1] 吴军林.L-苹果酸保健功能评价及作用机理研究[D].北京:中国科学院研究生院, 2007

[2]梅郁, 张缨.苹果酸代谢与运动[J].中国运动医学杂志, 2005, 24(4):509-511

[3] SCHOLZ TD, TENEYCK CJ, SCHUTTE BC.Thyroid hormone regulation of the NADH shuttles in liver and cardiac mitochondria[J]. Mol Cell Cardiol. 2000.2(1):1-10.[J].J Mol Cell Cardiol, 2000, 32:1-10

[4] 朱瑞娟, 饶鑫峰, 魏登邦, 等.高原鼢鼠和高原鼠兔肝脏苹果酸天冬氨酸穿梭系统的功能差异[J]. 生理学报2012, 64(2): 177–186.[J].生理学报, 2012, 64(2):177-186

[5] 肖春.食药用菌多糖降血糖作用机理研究[D].北京:中国科学院研究生院, 2012.

[6] LIVAK KJ, SCHMITTGEN TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ct method[J]. Methods, 2001.25(4): 402-408.[J].Methods, 2001, 25(4):402-408

[7] LI Hui, JIANG Tao, LIN Yifeng, et al.HGF Proteets rat mesangial cells from high glueose一mediated oxidative stress[J]. Am J Nephrol 2006.26:519–530[J].Am J Nephrol, 2006, 26:519-530

[8]朱华, 吴耀生.实时荧光定量PCR检测三七SS基因表达的初步实践[J].广西植物, 2008.28(5):703-707[J].广西植物, 2008, 28(5):703-707

[9]SCHMITTGEN TD, LIVAK KJ.Analyzing real-time PCR data by the comparative CT method[J].Nature Protocol, 2006, 3(6):1101-1108

[10] RUI Shi, CHIANG VL.Facile means for quantifying microRNA expression by real-time PCR[J]. Bio Techniques 2005, 39:519-525[J].Bio Techniques, 2005, 39:519-525

[11] WU JunLin, WU QingPing, PENG Yunping, et.al. Effects of L-malate on mitochondrial oxidoreductases in liver of aged rats[J]. Physiol Res 2011; 60: 329–336[J].Physiol. Res, 2011 , 60:329-336

[12] WU JunLin, WU QingPing, HUANG Junming, et.al. Effects of L-malate on physical stamina and activities of enzymes related to the malate-aspartate shuttle in liver of mice[J]. Physiol. Res. 2007, 56: 213-220[J].Physiol. Res. 56: 213-220, 2007, 2007, 56:213-220

[13] WU JunLin, WU QingPing, YANG Xinfen.et.al L-Malate Reverses Oxidative Stress and Antioxidative Defenses in Liver and Heart of Aged Rats[J] Physiol. Res. 2008, 57: 261-268[J].Physiol. Res., 2008, 57:261-268

[14]ETO K, SUGA S, WAKUI M.et alNADH shuttle system regulates K (ATP) channel-dependent pathway and steps distal to cytosolic Ca(2+) concentration elevation in glucose-induced insulin secretion[J].Biol Chem, 1999, 274(36):25386-25392

[15]BARRON JT, GU Liping, PARRILLO JE.Malate-aspartate shuttle,cytoplasmic NADH redox potential,and energetics in vascular smooth muscle[J].J Mol Cell Cardiol, 1998, 30(8):1571-1579

[16] MITCHELL M, CASHMAN KS, GARDNER DK, et al.Disruption of Mitochondrial Malate-Aspartate Shuttle Activity in Mouse Blastocysts Impairs Viability and Fetal Growth. Biology of Reproduction, 2009. 80(2): 295-301.[J].BIOLOGY OF REPRODUCTION, 2009, 80:295-301

[17] KUNZ WS, DAVIS EJ.Control of reversible intracellular transfer of reducing potential[J].Arch Biochem Biophys, 1991.284:40-46.[J].ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 284:40-46

[18] LANOUE KF, TISCHLER ME.Electrogenic characteristics of the mitochondrial glutamate-aspartate antiporter[J]. J BiolChem, 1974, 249:7522-7528[J].J. Biol. Chem, 1974, 249:7522-7528

[19] SCHOLZ TD, GRIER BL, THOMPSON GS, et al.Developmental regulation of the α-glycerophosphate shuttle in porcine myocardium. J Mol Cell Cardiol 1997; 29: 1605–1613[J].J Mol Cell Cardiol, 1997, 29:1605-1613

[20] 吴军林, 吴清平, 韦明肯, 等.L-苹果酸对苹果酸天冬氨酸穿梭转运蛋白及酶基因表达的作用研究[J]食品科学, 2006, 27（11）:229-232[J].食品科学, 2006, 27(11):229-232

[21] SCHOLZ.TD, KOPPENHAFER.SL, TENEYCK.CJ, et al. Ontogeny of malate-aspartate shuttle capacity and gene expression in cardiac mitochondria[J]. Am J Physiol Cell Physiol 1998, 274:C780-C788[J].Am J Physiol Cell Physiol, 1998, 274:780-788

L-苹果酸对大鼠转运蛋白及抗氧化酶基因表达的影响

Effect of L-malate on Gene Expression of Carrier Proteins and Antioxidant Enzymes in Rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	潘涛林金德余颖豪郭丽琼林俊芳. 酿酒酵母表达侧耳源单基因生物合成麦角硫因[J]. 食品科学, 0, (): 0-0.
[2]	刘乐丁一王紫玄房耀维王淑军吕明生. 海洋氧化节杆菌KQ11右旋糖酐酶催化位点关键氨基酸研究[J]. 食品科学, 0, (): 0-0.
[3]	刘乐，丁一，王紫玄，房耀维，王淑军，吕明生. 海洋氧化节杆菌KQ11右旋糖苷酶催化位点关键氨基酸[J]. 食品科学, 2019, 40(6): 113-120.
[4]	费理文王勇. 构建高效糖配体再生重组菌生物催化合成莱鲍迪苷D[J]. 食品科学, 0, (): 0-0.
[5]	赵齐冯志彬秦雪徐勤省任梦云潘潇张洪霞张娟. 特基拉芽孢杆菌精氨酸酶基因的重组表达及酶学性质[J]. 食品科学, 0, (): 0-0.
[6]	赵齐，冯志彬，秦雪，徐勤省，任梦云，潘潇，张洪霞，张娟. 特基拉芽孢杆菌精氨酸酶基因的重组表达及酶学性质[J]. 食品科学, 2018, 39(8): 77-82.
[7]	黄钦耿梁玲陈巧红吴松刚黄建忠. 小菜蛾moricin在毕赤酵母中的表达及抑菌活性分析[J]. 食品科学, 0, (): 0-0.
[8]	黄钦耿，梁玲，陈巧红，吴松刚，黄建忠. 小菜蛾moricin在毕赤酵母中的表达及抑菌活性分析[J]. 食品科学, 2017, 38(16): 36-42.
[9]	崔青钱炳俊季顺利姚晓敏张建华. 纳豆激酶基因工程菌构建及酶活性分析[J]. 食品科学, 0, (): 0-0.
[10]	张明哲. 复合PCR扩增荧光检测方法在转基因水稻品系检测中的应用研究[J]. 食品科学, 0, (): 0-0.
[11]	陈小举，操新民，姜绍通，李兴江. 氧化还原电位调控对钝齿棒杆菌代谢通量分布的影响[J]. 食品科学, 2016, 37(9): 165-169.
[12]	赵新宇陈杨阳陈敬帮蔡冬波魏雪团. 高产纳豆激酶地衣芽胞杆菌工程菌全合成培养基优化[J]. 食品科学, 0, (): 0-0.
[13]	赵新宇，陈杨阳，陈敬帮，蔡冬波，魏雪团. 高产纳豆激酶地衣芽孢杆菌工程菌全合成培养基优化[J]. 食品科学, 2016, 37(7): 140-145.
[14]	陈敬帮周银华赵新宇陈守文魏雪团. α-淀粉酶在不同地衣芽孢杆菌宿主菌中分泌表达的对比分析[J]. 食品科学, 0, (): 0-0.
[15]	陈敬帮，周银华，赵新宇，陈守文，魏雪团. α-淀粉酶在不同地衣芽孢杆菌宿主菌中分泌表达的对比分析[J]. 食品科学, 2015, 36(17): 196-200.