人参糖肽结合有氧运动对自发性糖尿病大鼠糖脂代谢的影响

doi:10.7506/spkx1002-6630-201419054

摘要/Abstract

摘要：

目的：研究人参糖肽（ginseng glycopeptides，GGP）结合有氧运动对自发性糖尿病（goto-kakisaki，GK）大鼠糖脂代谢的影响。方法：将40 只GK大鼠随机分为对照组（C组）、GGP 80 mg/（kg·d）组（G组）、有氧运动组（E组）、GGP 80 mg/（kg·d）组＋有氧运动组（GE组）。E组大鼠每天进行无负重游泳60 min，每周训练5 d，共8 周。期间G组按80 mg/（kg·d）皮下注射给药，GE组按80 mg/（kg·d）皮下注射给药GGP（每天先进行无负重游泳60 min，然后立即给药），C组皮下注射给予同体积生理盐水，每天一次，连续8 周。每笼一只，每周称量体质量、进食量、饮水量、排尿量、测血糖一次，末次训练和给药结束后，禁食12 h，乙醚麻醉，腹主动脉取血，检测GK大鼠血清中血糖（glucose，GLU）、胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）、胰岛素（serum insulin，INS）、C肽（C-peptide，C-P）、肿瘤坏死因子（tumor necrosis factor-α，TNF-α）、游离脂肪酸（free fatty acid，FFA）及一氧化氮（NO）水平。结果：C组、E组、G组和GE组对GK大鼠体质量增长和进食量组间比较均无明显差异（P＞0.05）。与C组比较，E组对GK大鼠饮水量和尿量有降低趋势，但无统计学差异（P＞0.05）；G组和GE组均可明显降低GK大鼠饮水量和尿量（P＜0.05或P＜0.01）；E组GK大鼠TC含量明显降低（P＜0.05），对其他指标均无统计学差异（P＞0.05）；G组GLU、TNF-α、FFA含量明显降低（P＜0.05），INS、C-P及NO水平则明显升高（P＜0.05），但对TC和TG含量则无明显统计学差异（P＞0.05）；GE组GLU、TC、TG、TNF-α、FFA水平明显降低（P＜0.05或P＜0.01），INS、C-P及NO水平则明显升高（P＜0.05）。与G组比较，GE组第1周饮水量和排尿量有明显差异（P＜0.05），其他各观察点有降低趋势但无统计学差异（P＞0.05），E组饮水量和排尿量均高于G组，但无明显差异（P＞0.05）；GE组GLU与TC含量较G组明显降低（P＜0.05），NO、INS及C-P水平较G组均有升高，TG、TNF-α、FFA含量均有降低，但上述指标均无统计学差异（P＞0.05）；E组TC及C-P水平明显降低（P＜0.05），NO及INS水平较G组均有降低，GLU、TG、TNF-α、FFA含量均有升高，而上述指标均无统计学差异（P＞0.05）。与E组比较，GE组排尿量与GLU含量明显降低（P＜0.05或P＜0.01），C-P含量明显升高（P＜0.05），但对其他指标均无显著性差异（P＞0.05）。结论：有氧运动与GGP结合可明显降低GK糖尿病大鼠血糖和血脂水平，改善糖脂代谢紊乱及胰岛素抵抗。

关键词: 人参糖肽, GK大鼠, 糖脂代谢, 胰岛素抵抗

Abstract:

Purpose: To study the effect of ginseng glycopeptide (GGP) combined with aerobic exercise on glucose and lipid
metabolism disorders in Goto-Kakisaki rats (GK rats). Methods: Totally 40 GK rats were randomly divided into four groups by
blood glucose levels as model control group (group C), 80 mg/(kg·d) GGP group (group G), aerobic exercise group (group E),
and 80 mg/(kg·d) GGP plus aerobic exercise group (group GE). GK rats in exercise group underwent 60 min swimming
without weight loading every day 5 days per week for 8 weeks. GGP preparation was subcutaneously injected to GK rats
in GGP group at the dose of 80 mg/(kg·d). The model control rats were treated with saline via subcutaneous injection for
8 weeks. Body weight, food and water intake, urine output, and serum glucose concentration were measured every week.
After the last training followed by 12 h fasting, glucose (GLU), triglyceride (TG), total cholesterol (TC), free fatty acid
(FFA), nitric oxide (NO), serum insulin (INS), C-peptide (C-P), and tumor necrosis factor-α (TNF-α) were also determined.
Results: Among all groups, the body weight and food intake of GK rats did not exhibit significant difference (P ＞ 0.05).
Compared with group C, group E showed lower but not significantly different water intake and urine output (P ＞ 0.05),
but a significant reduction in both parameters was observed for groups G and GE (P ＜ 0.05 or P ＜ 0.01). The GK rats
from group E showed a significant reduction in serum TC level (P ＜ 0.05), while showing no significant difference in all
other indicators (P ＞ 0.05). The levels of GLU, TNF-α and FFA in group G were significantly lower than those in group C
(P ＜ 0.05), and the levels of INS C-P and NO were significantly higher than those in group C (P ＜ 0.05), while there was
no significant difference in serum TC or TG levels (P ＞ 0.05). Group GE significantly reduced the levels of GLU, TC, TG,
TNF-α and FFA (P ＜ 0.05 or P ＜ 0.01), and significantly increased the levels of INS, C-P and NO (P ＜ 0.05). Compared
with group G, the water intake and urine output in group GE were significantly reduced after the first week (P ＜ 0.05), and
tended to be lower but not significantly different at other time points (P ＞ 0.05). The water intake and urine output in group
E were higher than but not significantly different from those in group G (P ＞ 0.05). The levels of GLU and TC in group GE
significantly reduced when compared with those in group C (P ＜ 0.05); however, the levels of NO, INS and C-P in group
GE were increased and the levels of TG, TNF-α and FFA were reduced but without significantly different (P ＞ 0.05). Group
E indicated a significant decrease in TC and C-P levels in comparison to group C (P ＜ 0.05), and presented lower NO and
INS levels and higher GLU, TG, TNF-α, and FFA levels but without showing significant difference (P ＞ 0.05). Group GE
exhibited a significant reduction in urine output and GLU level (P ＜0.05 or P ＜ 0.01), a significant increase in C-P level
(P ＜ 0.05), but no significant differences in all other parameters (P ＞ 0.05) compared with group E. Conclusion: GGP
combined with can significantly decrease the levels of blood glucose and blood lipid in GK rats, improve the metabolism
disorders of glucose and lipid, and ameliorate insulin resistance.

Key words: ginseng glycopeptide, goto-kakisaki rat, metabolism disorders of glucose and lipid, insulin resistance

中图分类号:

R285.5

刘雪梅，杨铭，于德伟，陈文学，杨明. 人参糖肽结合有氧运动对自发性糖尿病大鼠糖脂代谢的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201419054.

LIU Xue-mei, YANG Ming, YU De-wei, CHEN Wen-xue, YANG Ming. Effect of Ginseng Glycopeptide (GGP) Combined with Aerobic Exercise on Glucose and Lipid Metabolism Disorders in Goto-Kakisaki Rats[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201419054.

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人参糖肽结合有氧运动对自发性糖尿病大鼠糖脂代谢的影响

Effect of Ginseng Glycopeptide (GGP) Combined with Aerobic Exercise on Glucose and Lipid Metabolism Disorders in Goto-Kakisaki Rats

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