食品科学

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4 ℃贮藏期内冷鲜羊肉表面菌相变化分析

周琰冰1,1,*,张德权2,*   

  1. 1.北京农学院食品科学与工程学院,北京 102206;2.中国农业科学院农产品加工研究所,北京 100193
  • 出版日期:2015-03-25 发布日期:2015-03-17
  • 通讯作者: 艾启俊
  • 基金资助:

    公益性行业(农业)科研专项(201303083)

Changes in Microflora on Fresh Mutton during Chilled Storage

ZHOU Yanbing1, AI Qijun1,*, ZHANG Dequan2,*   

  1. 1. College of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China;
    2. Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Online:2015-03-25 Published:2015-03-17
  • Contact: AI Qijun

摘要:

研究4 ℃条件下贮藏时间对冷鲜羊肉表面菌相组成的影响,以菌落总数为指标,结合菌落聚合酶链式反应法,了解冷鲜羊肉在其贮藏期内的菌群构成,以及随着贮藏时间延长其优势菌群的变化。结果表明,冷鲜羊肉在4 ℃托盘贮藏条件下,菌落总数随贮藏时间的延长而增加,第7天其菌落总数超过106 CFU/g,样品已经腐败变质;其表面主要菌群包括假单胞菌属、芽孢杆菌属、不动杆菌属等。随着贮藏时间的变化,假单胞菌占总菌相的大部分,是造成冷鲜羊肉腐败变质的主要优势菌。

关键词: 冷鲜羊肉, 菌落总数, 菌落聚合酶链式反应, 4 ℃冷藏, 优势菌

Abstract:

The composition of the bacterial microflora on fresh mutton as a function of storage time at 4 ℃ was determined
by total plate count and colony polymerase chain reaction (PCR). The results showed that the total number of colonies on
tray-packaged mutton increased with an increase in storage time and exceeded 106 CFU/g at the seventh day, indicating
spoilage of the mutton. The main bacteria were Pseudomonas, Bacillus, Acinetobacter and etc. During storage, Pseudomonas
became the dominant spoilage bacterium in chilled meat.

Key words: chilled fresh mutton, total number of colonies, colony PCR, refrigeration at 4 ℃, dominant bacteria

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