食品科学

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响应面试验优化丹参中多糖的超声波提取工艺及其抗氧化活性

王燕华,武福华,郭昭涵,彭明星,宋万华,夏 敏,梁子安,张乃群   

  1. 南阳师范学院生命科学与技术学院,河南 南阳 473061
  • 出版日期:2015-09-25 发布日期:2015-09-11
  • 基金资助:

    国家自然科学基金青年科学基金项目(11102092);河南省教育厅自然基金项目(12B130003);
    河南省生物化学与分子生物学重点学科项目

Optimization of Ultrasonic-Assisted Extraction and Antioxidant Activities of Polysaccharides from the Roots of Salvia miltiorrhiza Bge.

WANG Yanhua, WU Fuhua, GUO Zhaohan, PENG Mingxing, SONG Wanhua, XIA Min, LIANG Zi’an, ZHANG Naiqun   

  1. College of Life Science, Nanyang Normal University, Nanyang 473061, China
  • Online:2015-09-25 Published:2015-09-11

摘要:

以丹参为原料,利用超声波提取丹参多糖。在单因素试验的基础上,应用Box-Behnken试验设计软件对超声时间、超声功率、颗粒大小工艺条件进行分析与优化。同时,以1,1-二苯基-2-三硝基苯肼自由基清除能力、增强内皮细胞内超氧岐化酶的能力评价超声波法提取丹参多糖的抗氧化活性。结果表明:超声波提取丹参多糖的最优提取条件为超声功率212 W、超声时间18 min、颗粒大小55 目,此条件下多糖提取率可达4.73%。抗氧化实验结果表明,丹参多糖有一定抗氧化活性。超声波浸提法相对单纯热水浸提法可以有效地缩短多糖提取时间,节约能源成本和时间,同时多糖活性更高。

关键词: 丹参, 超声提取, 多糖, 响应面优化, 抗氧化

Abstract:

The ultrasonic-assisted extraction of polysaccharides from the roots of Salvia miltiorrhiza with respect to three
process parameters including ultrasonication time, ultrasonic power and raw material particle size was optimized using
combination of single factor experiments and Box-Behnken design. Meanwhile the antioxidant activity in vitro of the
extracted polysaccharides was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability. The ability to
repair endothelial injury induced by high sugar content was analyzed by superoxide dismutase (SOD) activity in endothelial
cells. The optimum extraction conditions that provided the maximum yield of polysaccharides of 4.73% were determined
as follows: ultrasonic power, 212 W; extraction time, 18 min; and raw material particle size, 55 mesh. Antioxidant assays
showed that the polysaccharides extracted from Salvia miltiorrhiza possessed antioxidant activity. Compared with hot
water extraction, ultrasound-assisted extraction could effectively shorten the extraction time, save energy costs and yield
polysaccharides with higher activity.

Key words: Salvia miltiorrhiza Bge., ultrasonic-assisted extraction, polysaccharide, response surface analysis, antioxidant activity

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