可视化蛋白芯片法同时检测牛乳中残留的磺胺类和喹诺酮类药物

doi:10.7506/spkx1002-6630-201602034

食品科学

可视化蛋白芯片法同时检测牛乳中残留的磺胺类和喹诺酮类药物

钟文英1，王兴如1，许丹科2,*，李钟卉2，李周敏2

1.中国药科大学理学院分析化学教研室，江苏南京 211198；
2.南京大学化学化工学院，生命分析化学国家重点实验室，江苏南京 210093

出版日期:2016-01-25 发布日期:2016-01-15
通讯作者: 许丹科
基金资助:
江苏研究生科研创新计划项目（KYZZ-0184）

Simultaneous Determination of Multiresidues of Sulfonamides and Quinolones in Milk with Visual Protein Chip

ZHONG Wenying1, WANG Xingru1, XU Danke2,*, LI Zhonghui2, LI Zhoumin2

1. Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 211198, China; 2. State Key Laboratory of
Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China

Online:2016-01-25 Published:2016-01-15
Contact: XU Danke

摘要/Abstract

摘要：

目的：利用可视化蛋白芯片分析技术对牛乳中磺胺类和喹诺酮类抗生素多残留的同时检测。方法：采用间接竞争法反应原理，将磺胺类和喹诺酮类药物的人工抗原以微阵列形式固定于微孔板底部，制备成微孔板生物芯片阵列，并依次与磺胺类和喹诺酮类单克隆抗体、纳米银标记羊抗鼠IgG反应，最后用纳米银增强法显色，并采用可视化芯片扫描仪对微孔板显色结果进行快速扫描成像，图像采用芯片分析软件处理。结果：本法磺胺嘧啶和恩诺沙星线性范围为分别为0.3～7.2 ng/mL和0.4～18 ng/mL。空白牛乳中加标磺胺嘧啶（0.5、2.0、5.0 ng/mL）和恩诺沙星（0.5、3.0、15.0 ng/mL），结果牛乳中2 种药物的加标回收率分别为83%～110%，且相对标准偏差均在10%以内。结论：本法能够用于牛乳样本中磺胺类和喹诺酮类药物的残留的快速初筛。

关键词: 蛋白质芯片, 间接竞争法, 多残留检测, 磺胺类药物, 喹诺酮类药物

Abstract:

Purpose: To develop a protein microarray method for the simultaneous detection of multiresidues of two
veterinary drugs, sulfonamides and quinolones, in milk with a visual protein chip. Methods: A 96-well microplate was
used as solid support, on which artificial antigens of sulfonamides and quinolones were immobilized, respectively. After
immobilization, a mixture of antibodies to two analytes and either standard solutions containing the analytes or samples were
added to the array reaction area. Silver nanoparticles (AgNPs)-labeled goat anti-mouse IgG were used as an indicator and
nanosilver enhancement technique was applied to amplify the detection signals, producing black image on array spots visible
with naked eyes. The signals were detected with a visual scanner; therefore the analyte residues could detected quantitatively.
Results: The linear ranges for sulfonamides and quinolones were 0.3–7.2 ng/mL and 0.4–18 ng/mL respectively. The
recovery rates were in the range of 83% to 110% for milk samples at spiked levels of 0.5/0.5, 2.0/3.0, and 5.0/15.0 ng/mL
with coefficient of variation (CV) values < 10%. Conclusions: These results indicate that the protein microarray method is
suitable for the routine screening of multiresidues of sulfonamides and quinolones in milk.

Key words: protein chip, indirect competition, multiresidue detection, sulfonamides, quinolones

中图分类号:

O657

钟文英，王兴如，许丹科，李钟卉，李周敏. 可视化蛋白芯片法同时检测牛乳中残留的磺胺类和喹诺酮类药物[J]. 食品科学, doi: 10.7506/spkx1002-6630-201602034.

ZHONG Wenying, WANG Xingru, XU Danke, LI Zhonghui, LI Zhoumin. Simultaneous Determination of Multiresidues of Sulfonamides and Quinolones in Milk with Visual Protein Chip[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201602034.

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可视化蛋白芯片法同时检测牛乳中残留的磺胺类和喹诺酮类药物

Simultaneous Determination of Multiresidues of Sulfonamides and Quinolones in Milk with Visual Protein Chip

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