菜籽多肽体外和细胞内抗氧化性评价及氨基酸分析

doi:10.7506/spkx1002-6630-201613007

食品科学

菜籽多肽体外和细胞内抗氧化性评价及氨基酸分析

张晶，张怡一，徐斐然，石嘉怿，鞠兴荣，王立峰

南京财经大学食品科学与工程学院，江苏省现代粮食流通与安全协同创新中心，江苏高校粮油质量安全控制及深加工
重点实验室，江苏南京 210023

出版日期:2016-07-15 发布日期:2016-07-26
通讯作者: 王立峰
基金资助:
国家重点研发计划项目（2016YFD0401401）；国家高技术研究发展计划（863计划）项目（2013AA102207）；
国家农业成果转化计划项目（2014GB2C100318）；江苏省产学研前瞻性联合研究项目（BY2016009-01）；
江苏省高校自然科学基金项目（14KJB550004）；江苏高校优势学科建设工程资助项目；
南京财经大学研究生科技创新计划资助项目

Antioxidant Activities in Vitro and in Cells and Amino Acid Composition of Rapeseed Peptides

ZHANG Jing, ZHANG Yiyi, XU Feiran, SHI Jiayi, JU Xingrong, WANG Lifeng

Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and
Processing, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China

Online:2016-07-15 Published:2016-07-26
Contact: WANG Lifeng

摘要/Abstract

摘要：

本研究以Alcalase 2.4L酶解“双低”油菜籽宁杂19号得到的菜籽蛋白酶解物（rapeseed protein hydrolysate，RPH）为原料。通过超滤和凝胶色谱分离其活性肽组分，对酶解液及各分离组分进行抗氧化活性研究，并对抗氧化能力最高的组分进行氨基酸分析。结果表明：通过超滤将RPH分离成4 组不同分子质量范围的多肽组分，其中RPH-P4（分子质量＜3 kD）具有最高的抗氧化活性；RPH-P4经Sephadex G-15凝胶柱分离后得到3 个洗脱峰，收集并测定其活性，研究发现RPH-P4-S3抗氧化能力远高于其他2 个组分（P＜0.05），氧自由基吸收能力（oxygenradical absorbance capacity，ORAC）、细胞抗氧化活性（cellular antioxidant activity，CAA）、细胞内CAA实验的EC50值分别为（1 951.90±20.35） μmol TE/g、（143.38±4.11） μmol QE/g、（45.63±3.67） μg/mL；对RPH-P4-S3进行了氨基酸分析，发现其抗氧化性氨基酸含量达到72.90%，必需氨基酸含量为53.52%。研究认为，菜籽蛋白Alcalase 2.4L酶解物分离组分RPH-P4-S3具有较高的抗氧化活性及营养价值，可以作为功能性成分用于抗氧化相关的功能食品和保健品的开发。

关键词: 菜籽多肽, 抗氧化能力指数, 细胞抗氧化能力, 氨基酸分析

Abstract:

The rapeseed protein hydrolysate (RPH) obtained by enzymatic hydrolysis of double-zero rapeseed with Alcalase
2.4L was separated by ultrafiltration and gel filtration chromatography to produce active peptide fractions. The antioxidant
activity of RPH and its active peptides was evaluated in vitro. Furthermore, the amino acid composition of the most potent
antioxidant fraction was analyzed. The results showed that RPH was separated into 4 fractions in different molecular weight
ranges. RPH-P4 (molecular weight < 3 kD) had the strongest antioxidant capacity among these peptide fractions. RPH-P4 was
further separated by gel filtration on Sephadex G-15 into 3 peaks. The antioxidant capacity of RPH-P4-S3 was far higher than
that of 2 other peaks (P < 0.05). Its oxygen radical absorbance capacity (ORAC) value was (1 951.90 ± 20.35) μmol TE/g
sample, cellular antioxidant activity (CAA) value (143.38 ± 4.11) μmol QE/g sample, and EC50 value (45.63 ± 3.67) μg/mL.
The content of antioxidant amino acids in RPH-P4-S3 was as high as 72.90%, and the essential amino acid content was
53.52%. This research suggests that RPH-P4-S3, produced by enzymatic hydrolysis of double-zero rapeseed with Alcalase
2.4L, has high antioxidant activity and nutritional value and thus can be applied in the development of antioxidant-related
functional foods and healthcare products as a functional ingredient.

Key words: rapeseed peptides, oxygen radical absorbance capacity, cellular antioxidant activity, amino acid analysis

中图分类号:

TS201.4

张晶，张怡一，徐斐然，石嘉怿，鞠兴荣，王立峰. 菜籽多肽体外和细胞内抗氧化性评价及氨基酸分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201613007.

ZHANG Jing, ZHANG Yiyi, XU Feiran, SHI Jiayi, JU Xingrong, WANG Lifeng. Antioxidant Activities in Vitro and in Cells and Amino Acid Composition of Rapeseed Peptides[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613007.

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菜籽多肽体外和细胞内抗氧化性评价及氨基酸分析

Antioxidant Activities in Vitro and in Cells and Amino Acid Composition of Rapeseed Peptides

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