关键词: 酿酒酵母, 5.8S-ITS区域RFLP分析, Interdelta PCR指纹图谱法, 多样性, 本土酵母

Abstract: In this study, the biodiversity of Saccharomyces cerevisiae isolated from different environments associated with Longan grape in Shacheng wine-producing region was investigated. In the continuous three years 2008, 2009 and 2010, 227 samples were collected from vineyard soil, the surface of winery equipments, grape juice contacted with winery equipments and naturally fermented grape and 1358 yeast strains were isolated from them. Molecular-level identification based on a PCR-RFLP analysis of 5.8S ITS rRNA and secondary screening using lysine medium were carried out to obtain 270 Saccharomyces cerevisiae strains. Further, the strains were divided using Interdelta PCR into 16 classes, including 5 from vineyard soil, 4, 10 and 11 from at the 2nd, 3rd and 4th stages of natural fermentation respectively, 3 from the surface of winery equipments and 3 from grape juice contacted with winery equipments. Saccharomyces cerevisiae species notably varied with sampling time and habitat. Saccharomyces cerevisiae strains isolated from naturally fermented grape had the greatest possibility of being indigenous strains.

Key words: Saccharomyces cerevisiae, restriction pattern of amplified 5.8S-ITS rRNA gene (RFLP), Interdelta PCR, biodiversity, indigenous yeast