日粮补充L-亮氨酸对超早期断奶宫内发育迟缓仔猪肠道免疫功能的影响

doi:10.7506/spkx1002-6630-201717030

食品科学 ›› 2017, Vol. 38 ›› Issue (17): 184-189.doi: 10.7506/spkx1002-6630-201717030

日粮补充L-亮氨酸对超早期断奶宫内发育迟缓仔猪肠道免疫功能的影响

黄强，徐稳，何进田，白凯文，苏伟鹏，周乐，张莉莉，王恬*

南京农业大学动物科技学院，江苏南京 210095

出版日期:2017-09-15 发布日期:2017-09-12
基金资助:
国家重点基础研究发展计划（973计划）项目（2012CB124703）

Effect of Dietary L-Leucine Supplementation on Intestinal Immune Function in Ultra-Early Weaned Piglets with Intrauterine Growth Retardation

HUANG Qiang, XU Wen, HE Jintian, BAI Kaiwen, SU Weipeng, ZHOU Le, ZHANG Lili, WANG Tian*

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Online:2017-09-15 Published:2017-09-12

摘要/Abstract

摘要： 目的：研究L-亮氨酸对超早期断奶宫内发育迟缓（intrauterine growth retardation，IUGR）仔猪肠道免疫功能的调节作用。方法：选取14 日龄断奶的正常初生体质量（normal birth weight，NBW）和IUGR新生仔猪各16 头，分别饲喂基础日粮或添加0.35% L-亮氨酸实验日粮，即4 个处理组，每组8 头猪。结果：与NBW仔猪相比，IUGR仔猪血清溶菌酶活力和补体3（complement-3，C3）水平显著降低（P＜0.05），空肠和回肠中杯状细胞（goblet cells，GC）和淋巴细胞（intraepithelial lymphocytes，IEL）数量显著减少（P＜0.05），回肠中肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）含量显著升高（P＜0.05），白细胞介素10（interleukin-10，IL-10）含量显著降低（P＜0.05）。另外，IUGR仔猪回肠TNF-α mRNA表达量显著升高（P＜0.05），IL-10 mRNA表达量显著降低（P＜0.05）。日粮添加0.35% L-亮氨酸后，IUGR仔猪血清溶菌酶活力、C3和C4水平显著升高（P＜0.05），回肠中GC数量及十二指肠和空肠中IEL数量显著增加（P＜0.05），回肠中IL-10含量显著升高（P＜0.05），并且IL-2和IL-10 mRNA表达量显著升高（P＜0.05）。结论：IUGR会引起超早期断奶仔猪肠道黏膜发生炎症反应和肠道生长发育受限。L-亮氨酸可以有效缓解IUGR引起的肠道黏膜损伤，改善仔猪肠道的免疫功能。

关键词: L-亮氨酸, 宫内发育迟缓, 肠道, 免疫功能, 早期断奶仔猪

Abstract: Objective: To ascertain the effect of dietary leucine supplementation on intestinal immune function in ultra-early weaned piglets suffering from intrauterine growth retardation (IUGR). Methods: Sixteen normal birth weight (NBW) piglets weaned at 14 days of age and sixteen IUGR newborn piglets were selected according to their birth weight. All the piglets were randomly divided into four groups with 8 replicates in each group, and fed control diet alone or supplemented with 0.35% L-leucine for 21 days, respectively. Results: Compared with NBW piglets, IUGR piglets showed significantly lower lysozyme activity and complement-3 (C3) level in the serum (P < 0.05), a significant reduction in the number of goblet cells (GC) and intraepithelial lymphocytes (IEL) in the jejunum and ileum (P < 0.05) and interleukin-10 (IL-10) content in the ileum, and a significant increase intumor necrosis factor-α (TNF-α) content in the ileum (P < 0.05). In addition, IUGR piglets had significantly increased TNF-α mRNA expression but significantly decreased IL-10 mRNA expression (P < 0.05). Dietary supplementation with 0.35% L-leucine significantly increased lysozyme activity and the levels of C3 and C4 in the serum, the numbers of GC in the ileum and IEL in the duodenum and jejunum, IL-10 content in the ileum, and the mRNA expression of IL-2 and IL-10 in the ileum of IUGR piglets (P < 0.05). Conclusion: IUGR could induce intestinal mucosal inflammatory response and hinder intestinal growth and development in early weaned piglets. L-Leucine could effectively alleviate intestinal mucosal injury caused by IUGR, and improve intestinal immune function in piglets.

Key words: L-leucine, intrauterine growth retardation (IUGR), intestine, immune function, early weaned piglets

中图分类号:

TS202.3

黄强，徐稳，何进田，白凯文，苏伟鹏，周乐，张莉莉，王恬. 日粮补充L-亮氨酸对超早期断奶宫内发育迟缓仔猪肠道免疫功能的影响[J]. 食品科学, 2017, 38(17): 184-189.

HUANG Qiang, XU Wen, HE Jintian, BAI Kaiwen, SU Weipeng, ZHOU Le, ZHANG Lili, WANG Tian. Effect of Dietary L-Leucine Supplementation on Intestinal Immune Function in Ultra-Early Weaned Piglets with Intrauterine Growth Retardation[J]. FOOD SCIENCE, 2017, 38(17): 184-189.

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日粮补充L-亮氨酸对超早期断奶宫内发育迟缓仔猪肠道免疫功能的影响

Effect of Dietary L-Leucine Supplementation on Intestinal Immune Function in Ultra-Early Weaned Piglets with Intrauterine Growth Retardation

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