食品科学 ›› 2017, Vol. 38 ›› Issue (23): 141-148.doi: 10.7506/spkx1002-6630-201723023

• 营养卫生 • 上一篇    下一篇

沙棘粕醇提取物对秀丽隐杆线虫的抗衰老功效及其机制

张佳婵1,王昌涛1,2,*,刘 瑶1,石秀芹1,赵 丹1,李 萌1,王成涛2,3,孙宝国2,3   

  1. 1.北京工商大学理学院,植物资源研究开发北京市重点实验室,北京 100048;2.北京工商大学 北京食品营养与人类健康高精尖创新中心,北京 100048;3.北京工商大学食品学院,食品添加剂与配料工程研究中心,北京 100048
  • 出版日期:2017-12-15 发布日期:2017-12-07
  • 基金资助:
    国家自然科学基金面上项目(31571801)

Anti-Aging Effect and Its Mechanism of Ethanol Extract from Sea Buckthorn Seed Meal in Caenorhabditis elegans

ZHANG Jiachan1, WANG Changtao1,2,*, LIU Yao1, SHI Xiuqin1, ZHAO Dan1, LI Meng1, WANG Chengtao2,3, SUN Baoguo2,3   

  1. 1. Beijing Key Laboratory of Plant Resources Research and Development, School of Science, Beijing Technology and Business University, Beijing 100048, China; 2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; 3. Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China
  • Online:2017-12-15 Published:2017-12-07

摘要: 以沙棘粕为研究对象,经乙醇提取、大孔吸附树脂纯化获得沙棘粕醇提取物(sea buckthorn seed extracts, SBSE)。以秀丽隐杆线虫为模式生物,研究SBSE的抗衰老作用及其可能机制。本研究考察了不同质量浓度范围内 SBSE对秀丽隐杆线虫生命周期、生殖能力和应激抵御能力的影响,探索了SBSE对线虫抗氧化酶活力以及衰老相关 基因表达水平的作用,从基因水平探索SBSE的抗衰老可能机制。结果:SBSE可以显著延长秀丽隐杆线虫的存活时 间(P<0.05),减轻脂褐素水平的积累;0.05 mg/mL SBSE处理后秀丽隐杆线虫子代数量为(246.40±21.11)个, 显著高于空白对照组的(205.80±20.42)个(P<0.05);对热应激和氧化应激有显著的防御效果(P<0.05);此 外,SBSE提高了秀丽隐杆线虫体内超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活力,降低了秀丽隐杆 线虫促衰老基因age-1、daf-2的表达水平,提高了抗衰老基因sir-2.1的表达。该结果表明SBSE或许通过提高抗氧化 酶活力以及改变衰老相关基因的表达水平来发挥抗衰老的功效。

关键词: 沙棘粕醇提取物, 秀丽隐杆线虫, 抗氧化酶, 抗衰老

Abstract: In the present study, the anti-aging effect of sea buckthorn seed extracts (SBSE) obtained by ethanol extraction and macroporous resin purification was evaluated using Caenorhabditis elegans as a model organism and its underlying mechanism was discussed as well. The effect of different concentrations of SBSE was investigated on the lifespan, reproductive capability, heat and oxidative stress defense of C. elegans. In order to explore the anti-aging mechanism of SBSE at the gene level, the activity of antioxidant enzymes and the expression levels of related genes were determined. The results indicated that SBSE could significantly prolong the lifespan of C. elegans (P < 0.05), and reduce the accumulation of lipofuscin. Meanwhile, the number of C. elegans offsprings treated with 0.05 mg/mL of SBSE (246.40 ± 21.11) was significantly bigger than the control (205.80 ± 20.42) (P < 0.05). SBSE could significantly enhance the heat and oxidative stress defense (P < 0.05). Furthermore, SBSE increased the activity of superoxide dismutase, catalase and glutathione peroxidase, down-regulated the pro-aging gene expression levels of age-1 and daf-2, and up-regulated the anti-aging gene expression levels of sir-2.1. Therefore, SBSE possess an anti-aging effect by increasing the activity of antioxidant enzymes and changing the expression of aging-related genes.

Key words: sea buckthorn seed extracts, Caenorhabditis elegans, antioxidant enzymes, anti-aging

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