食品科学 ›› 2020, Vol. 41 ›› Issue (13): 127-132.doi: 10.7506/spkx1002-6630-20190626-351

• 营养卫生 • 上一篇    下一篇

辣木源多肽组分改善D-半乳糖致衰小鼠氧化损伤作用

耿春阳,林恋竹,赵谋明,朱启源   

  1. (华南理工大学食品科学与工程学院,广东 广州 510641)
  • 出版日期:2020-07-15 发布日期:2020-07-29
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400200)

Peptide Fractions from Moringa oleifera Ameliorate D-Galactose-Induced Oxidative Damage in Mice

GENG Chunyang, LIN Lianzhu, ZHAO Mouming, ZHU Qiyuan   

  1. (School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)
  • Online:2020-07-15 Published:2020-07-29

摘要: 为评价辣木源多肽组分在动物体内的抗氧化功效,本实验采用酶法、乙醇分级沉淀法制备辣木叶多肽组分(MOL-P)、辣木籽多肽组分(MOS-P),构建D-半乳糖致衰小鼠模型,考察MOL-P和MOS-P在低、高剂量(200、500 mg/(kg mb·d))下连续灌胃小鼠8 周,对衰老小鼠体质量,肝脏和脑的脏器系数,血清、肝脏和脑抗氧化酶活力和丙二醛(malondialdehyde,MDA)水平的影响。结果表明:MOL-P和MOS-P均能上调衰老小鼠脑系数至正常组水平(P>0.05);辣木源多肽组分能显著提高衰老小鼠血清谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力和肝脏超氧化物歧化酶(superoxide dismutase,SOD)活力(P<0.05),一定程度上降低血清、肝脏和脑组织MDA水平,其中低剂量MOL-P和高剂量MOS-P对血清GSH-Px活力的提升作用最好(分别比模型组提高了35.80%和36.97%);MOS-P对肝脏SOD活力提升作用最好,高、低剂量组分别比模型组提高了26.08%和34.20%;高剂量MOL-P和高剂量MOS-P对脑组织脂质过氧化的抑制作用最好(高剂量MOL-P和高剂量MOS-P脑组织MDA水平分别比模型组降低了41.28%和44.95%);MOL-P能将血清、肝脏MDA水平下调至正常组水平(P>0.05)。采用苏木精-伊红染色观察衰老小鼠肝脏组织损伤程度,结果表明,MOL-P和MOS-P均能有效缓解D-半乳糖所致肝脏氧化损伤。结论:辣木叶、辣木籽多肽组分均能在动物体内拮抗氧化应激,具有良好的抗衰老活性,可作为功能性因子用于抗衰老食品的研制。

关键词: 辣木叶, 辣木籽, 多肽, 氧化应激, 抗衰老

Abstract: In order to evaluate the antioxidant activity of peptide fractions from Moringa oleifera, peptides from Moringa oleifera leaves (MOL-P) and seeds (MOS-P) were prepared by enzymatic hydrolysis followed by fractional ethanol precipitation. A D-galactose-induced aging mouse model was established to assess the effects of administration with MOL-P and MOS-P at low and high doses (200 and 500 mg/(kg mb·d) by gavaging for 8 weeks on body mass, liver and brain indices, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) levels in serum, liver and brain of aging mice. The results indicated that MOL-P and MOS-P could restore brain indices close to normal (P > 0.05). Compared with the model group, both of them could significantly improve GSH-Px activity in serum and SOD activity in liver (P < 0.05), and reduce MDA levels in serum, liver and brain. Low-dose MOL-P and high-dose MOS-P had the best effects on improving GSH-Px activity in serum (higher than that of the model group by 35.80% and 36.97%, respectively). MOS-P had the best effect on the improvement of liver SOD activity, at high and low doses increasing liver SOD activity by 26.08% and 34.20% compared with the model group, respectively. MOL-P and MOS-P both at high dose showed the strongest inhibitory effects on lipid peroxidation in brain tissue (MDA levels were lower than that of the model group by 41.28% and 44.95%, respectively). In addition, MOL-P was able to reduce MDA levels in serum and liver to the normal level (P > 0.05). Both MOL-P and MOS-P could effectively alleviate oxidative damage in liver caused by D-galactose as evaluated by hematoxylin-eosin (HE) staining. Therefore, the peptides from Moringa oleifera leaves and seeds can antagonize oxidative stress in vivo and have good anti-aging effects, making them potential functional ingredients in anti-aging foods.

Key words: Moringa oleifera leave, Moringa oleifera seeds, peptide, oxidative stress, anti-aging

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