食品科学 ›› 2018, Vol. 39 ›› Issue (2): 260-266.doi: 10.7506/spkx1002-6630-201802041

• 工艺技术 • 上一篇    下一篇

微波辅助水酶法优化红枣中环磷酸腺苷(cAMP)提取工艺及其抗疲劳作用

任彦荣1,2,张忠明3,谢跃杰1,熊政委1,2,王仲明1,吴洪斌4,*   

  1. (1.重庆第二师范学院生物与化学工程学院,重庆 400067;2.重庆第二师范学院 重庆市功能性食品协同创新中心,重庆 400067;3.甘肃农业大学食品科学与工程学院,甘肃?兰州 737100;4.新疆农垦科学院农产品加工研究所,新疆?石河子 832000)
  • 出版日期:2018-01-25 发布日期:2018-01-05
  • 作者简介:任彦荣,张忠明,谢跃杰,熊政委,王仲明,吴洪斌
  • 基金资助:
    新疆建设兵团工业科技攻关项目(2015AB030);重庆市五大功能区域创新创业团队建设项目(201618793)

Optimization of Microwave-Assisted Aqueous Enzymatic Extraction of Cyclic Adenosine 3’,5’-Monophosphate (cAMP) from Chinese Jujube (Ziziphus jujuba Mill.) and Its Anti-Fatigue Effect

REN Yanrong1,2, ZHANG Zhongming3, XIE Yuejie1, XIONG Zhengwei1,2, WANG Zhongming1, WU Hongbin4,*   

  1. (1. College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China; 2. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China; 3. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 737100, China; 4. Institute of Agro-Food Science and Technology, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China)
  • Online:2018-01-25 Published:2018-01-05

摘要: 以新疆红枣为原料提取环磷酸腺苷(cyclic adenosine 3’,5’-monophosphate,cAMP),通过响应面法优化微波辅助复配酶法提取cAMP工艺,并建立回归模型;同时研究cAMP提取物对D-半乳糖诱导衰老模型小鼠的游泳耐力的影响。结果表明,最佳提取工艺为复配酶(纤维素酶-半纤维素酶-果胶酶)质量比1∶1∶1、复配酶添加量1.5%(质量分数)、酶解温度31.3?℃、酶解时间20?min、微波功率500?W,在此条件下cAMP提取量为560.9?μg/g;新疆红枣cAMP提取物能延长小鼠力竭游泳时间,增加小鼠游泳耐力,说明cAMP对小鼠具有很好的抗疲劳作用。新疆红枣作为cAMP的提取原料在食品和医药工业中具有较好的应用前景。

关键词: 红枣, 环磷酸腺苷(cAMP), 微波辅助水酶法提取, 游泳耐力

Abstract: Response surface methodology was used to optimize the microwave-assisted aqueous enzymatic extraction of cyclic adenosine 3’,5’-monophosphate (cAMP) from Chinese jujube (Zizyphus jujuba Mill.) grown in Xinjiang. The anti-fatigue effect of the cAMP extracted was investigated. The optimal extraction conditions was found as follows: enzyme concentration 1.5% (a mixture of cellulase, hemicellulase and pectinase, 1:1:1 (m/m)), temperature 31.3 ℃, time 20 min and microwave power 500 W. Under these conditions, the yield of cAMP was 560.9 μg/g. The results showed that the cAMP could extend exhaustive swimming time and enhance swimming endurance in mice, suggesting good anti-fatigue effect. Therefore, cAMP holds great promise for its application in the food and pharmaceutical industry.

Key words: Zizyphus jujuba, cyclic adenodsine 3’,5’-monophosphat (cAMP), microwave-assisted aqueous enzymatic extraction, swimming time

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