食品科学 ›› 2011, Vol. 32 ›› Issue (10): 57-60.doi: 10.7506/spkx1002-6630-201110013

• 工艺技术 • 上一篇    下一篇

超声波预处理微波萃取葛根总黄酮的工艺优化

顾仁勇,朱爱华,王文熙,刘 杰,李佑稽   

  1. 吉首大学化学化工学院
  • 出版日期:2011-05-25 发布日期:2011-04-08

Process Optimization for Extraction of Total Flavonoids from Radix Pueraria by Ultrasonic Followed by Microwave Treatment

GU Ren-yong,ZHU Ai-hua,WANG Wen-xi,LIU Jie,LI You-ji   

  1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China
  • Online:2011-05-25 Published:2011-04-08

摘要: 以湘西野葛根为原料,优化葛根总黄酮超声微波双辅助萃取工艺。研究超声提取温度、超声作用时间、微波功率、微波作用时间、固液比5个影响因素对葛根总黄酮提取量的影响,采用紫外分光光度计测定葛粉中总黄酮的含量,建立葛根总黄酮的最佳提取工艺条件。结果表明,在以80%的乙醇为提取溶剂、微波辐射功率300W、微波辐射时间9min、固液比1:25(g/mL)、超声提取温度50℃、超声提取时间30min的条件下,总黄酮的提取量可达14.685mg/g。

关键词: 葛根, 总黄酮, 超声波, 微波

Abstract: Ultrasonic followed by microwave treatment was used to establish a novel method to extract total flavonoids from Radix Pueraria. The effects of five extractions, namely temperature during ultrasonic treatment, ultrasonic treatment time, microwave power and treatment time and solid-to-liquid ratio on the extraction yield of total flavonoids from Radix Pueraria were explored. The quantification of total flavonoids was conducted using a UV spectrophotometer. The results showed that the maximum yield of total flavonoids could reach 14.685 mg/g was attained by using 80% ethanol as the extraction solvent at a solid-to-liquid ratio of 1:25 under the assistance of 30 min ultrasonic at 50 ℃ followed by 9 min microwave treatment at 300 W power.

Key words: Radix Pueraria, flavonoids, ultrasonic, microwave

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