食品科学 ›› 2011, Vol. 32 ›› Issue (11): 157-160.doi: 10.7506/spkx1002-6630-201111034

• 生物工程 • 上一篇    下一篇

冠突散囊菌发酵液的抗菌作用

李佳莲,胡博涵,赵勇彪,刘素纯,*,姜越君,刘仲华   

  1. 1.湖南农业大学食品科技学院 2.吉首大学化学化工学院 3.湖南省发酵食品工程技术研究中心 4.湖南农业大学 茶学教育部重点实验室
  • 出版日期:2011-06-15 发布日期:2011-05-13
  • 基金资助:
    湖南省重大科技专项(2008FJ1005);湖南省研究生创新课题(CX2010B282)

Anti-microbial Activity of Fermentation Supernatants of Eurotium cristatum

LI Jia-lian1,HU Bo-han1,ZHAO Yong-biao2,LIU Su-chun1,3,*,JIANG Yue-jun1,LIU Zhong-hua4   

  1. 1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. College of Chemistry and Chemical Engineering, Jishou Universitry, Jishou 416000, China; 3. Hunan Provincial Engineering and Technology Research Center for Fermented Food, Changsha 410128, China; 4. Key Laboratory of Tea Science, Ministry of Education, Hunan Agricultural University, Changsha 410128, China
  • Online:2011-06-15 Published:2011-05-13

摘要: 采用滤纸片法研究茯砖茶浸提液、PDA原液和冠突散囊菌PDA发酵液对细菌、酵母菌、霉菌和放线菌的抑制作用及酸碱度、温度和紫外光照射对冠突散囊菌PDA发酵液抑菌作用的影响。结果表明:茯砖茶浸提液和冠突散囊菌发酵液对细菌有较强的抑制作用,而对酵母菌和霉菌抑制作用不明显。通过对冠突散囊菌PDA发酵液抑菌物质稳定性的初步研究,结果表明,发酵液中的抑菌物质对温度(40~120℃)、紫外光(照射10h之内)和酸碱(pH2~10)稳定。

关键词: 冠突散囊菌发酵液, 抗菌作用, 稳定性

Abstract: The anti-microbial activities of hot water extract from Poria brick tea, PDA liquid medium and their fermentation supernatants by Eurotium cristatum against bacteria, fungi, mold and actinomycetes were determined by filter paper disc method. Meanwhile, the effects of factors including pH, temperature, UV on the anti-microbial activities of PDA fermentation supernatant were explored. The results indicated that both fermentation supernatants had an obvious anti-bacterial activity but no obvious inhibition effect against yeast and mold. Preliminary studies on the anti-microbial stability of the PDA fermentation supernatant showed that it had better stability under each of the following conditions: 40-120 ℃, UV irradiation for 10 h and pH 2-10.

Key words: Eurotium cristatum, fermented liquid, anti-microbial activity, stability

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