食品科学 ›› 2011, Vol. 32 ›› Issue (12): 73-77.doi: 10.7506/spkx1002-6630-201112017

• 工艺技术 • 上一篇    下一篇

米渣蛋白脱色工艺优化及对产品功能性质的影响

王毅梅,陈季旺,潘 浩,吴永宁   

  1. 1.武汉工业学院食品科学与工程学院 2.中国疾病预防控制中心营养与食品安全所
  • 出版日期:2011-06-25 发布日期:2011-06-10
  • 基金资助:
    国家“863”重点计划项目(2010AA023003);湖北省教育厅中青年人才项目(Q20091808);      武汉工业学院研究生创新基金项目(08cx021)

Optimum Decolorization Conditions and Functional Properties of Rice Residue Protein

WANG Yi-mei1,CHEN Ji-wang1,*,PAN Hao1,WU Yong-ning1,2   

  1. 1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2. Institute of Nutrition and Food Safety, Chinese Centre for Disease Control and Prevention, Beijing 100021, China
  • Online:2011-06-25 Published:2011-06-10

摘要: 为改善米渣蛋白的色泽和功能性质,采用响应面分析法对其脱色工艺条件进行优化,并分析脱色后米渣蛋白的功能性质。优化后双氧水脱色的较佳工艺条件为H2O2体积分数4.5%、pH9.5、温度55℃、时间45min。在此条件下,米渣蛋白的白度由20.5%增加到48.2%。在pH4~5时脱色后米渣蛋白的溶解度最低,只有5%左右;当pH<4或pH>6时,溶解度迅速增加,pH2和pH13时分别为38.58%和76.79%。中性条件下,脱色后米渣蛋白的起泡力12%、泡沫稳定性33.3%、乳化活力指标3.15、乳化稳定性指标24.84min、持油力和持水力分别为2.00和3.08,都明显高于未脱色米渣蛋白,说明米渣蛋白经H2O2氧化脱色后,可改善其功能性质。

关键词: 米渣蛋白, 双氧水, 脱色, 白度, 响应面分析法

Abstract: In order to improve the color and functional properties of rice residue protein, response surface methodology was used to optimize parameters influencing its decolorization by hydrogen peroxide. The optimum decolorization conditions were hydrogen peroxide. concentration of 4.5%, pH 9.5, decolorization temperature of 55 ℃ and decolorization time of 45 min. Under these conditions, the whiteness of rice residue protein was improved from 20.5% to 48.2%. In addition, the lowest solubility (roughly 5%) of rice residue protein in pH 4-5 was observed. However, when pH was lower than 4 or higher than 6, the solubility of rice residue protein was significantly increased. The solubility of rice residue protein was 38.58% at pH 2 or 76.79% at pH 13. In neutral environment, the foaming capacity (FC), foam stability (FS), emulsifying activity index (EAI), emulsifying stability index (ESI), oil-holding capacity (OHC) and water-holding capacity (WHC) of rice residue protein after decolorization were 12%, 33.3%, 3.15 min, 24.84 min, 2.00 and 3.08, respectively. All of these values were much higher than those before decolorization. Therefore, hydrogen peroxide treatment can not only decolorize rice residue protein but also significantly improve its functional properties.

Key words: rice residue protein, hydrogen peroxide, decoloration, whiteness, response surface methodology

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