食品科学

• 生物工程 • 上一篇    下一篇

暗纹东方鲀肌肉组织蛋白酶B提取工艺优化

李 琳,王正全,张晶晶,苗晓丹,马 垒,王锡昌,刘 源*   

  1. 上海海洋大学食品学院,上海水产品加工及贮藏工程技术研究中心,上海 201306
  • 出版日期:2016-02-15 发布日期:2016-02-26

Optimization of Extraction Conditions for Cathepsin B from Takifugu obscures Muscle

LI Lin, WANG Zhengquan, ZHANG Jingjing, MIAO Xiaodan, MA Lei, WANG Xichang, LIU Yuan*   

  1. Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2016-02-15 Published:2016-02-26

摘要:

为获得暗纹东方鲀组织蛋白酶B的最佳提取工艺条件,在单因素试验的基础上,通过Plackett-Burman设计对影响组织蛋白酶B活力的因素进行筛选,结果表明:料液比、磷酸盐缓冲液pH值和硫酸铵饱和度这3 个因素显著影响酶活力,然后用响应面分析法确定了主要影响因素的最佳提取条件为:料液比1∶2.1、磷酸盐缓冲液pH 7.0、硫酸铵饱和度99.2%,在此条件下组织蛋白酶B活力的预测值为32.65 U/g,实测值为32.52 U/g,相对误差为0.39%。说明建立的模型切实可行,为进一步研究组织蛋白酶B的提取工艺提供理论依据。

关键词: 暗纹东方鲀, 组织蛋白酶B, Plackett-Burman设计, 响应面分析法

Abstract:

In order to obtain optimal conditions for the extraction of cathepsin B from Takifugu obscures, on the basis of
results of single-factor experiments, some extraction parameters affecting cathepsin B activity were firstly evaluated by
Plackett-Burman design, and then the main variables were optimized by response surface analysis. The results showed that
the optimal extraction conditions were as follows: material/liquid ratio 1:2.1, phosphate buffer pH 7.0, and ammonium
sulfate saturation 99.2%, and these three factors had significant influence on the enzyme activity. Under these conditions,
the predicted value of cathepsin B activity was 32.65 U/g and its experimental value was 32.52 U/g, suggesting that the
established model is feasible and can provide a theoretical basis for further studies on the extraction process of cathepsin B.

Key words: Takifugu obscures, cathepsin B, Plackett-Burman design, response surface analysis

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