大豆蛋白与大豆低聚肽对负氮平衡老年小鼠表皮创伤感染下的免疫调节作用

doi:10.7506/spkx1002-6630-201817024

食品科学 ›› 2018, Vol. 39 ›› Issue (17): 145-151.doi: 10.7506/spkx1002-6630-201817024

大豆蛋白与大豆低聚肽对负氮平衡老年小鼠表皮创伤感染下的免疫调节作用

张健1，李雯晖1，赵博雅1，李赫1，刘新旗1,*，肖林2

1.北京食品营养与人类健康高精尖创新中心，北京市食品添加剂工程技术研究中心，北京工商大学，北京 100048；2.山东龙力生物科技股份有限公司，山东禹城 251200

出版日期:2018-09-15 发布日期:2018-09-18
基金资助:
“十三五”国家重点研发计划重点专项（2016YFD0400401）

Immune Modulation of Soybean Protein and Soybean Oligopeptides on Epidermal Trauma Infection in Aged Mice with Negative Nitrogen Balance

ZHANG Jian1, LI Wenhui1, ZHAO Boya1, LI He1, LIU Xinqi1,*, XIAO Lin2

1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; 2. Shandong Longlive Bio-technology Co. Ltd., Yucheng 251200, China

Online:2018-09-15 Published:2018-09-18

摘要/Abstract

摘要： 目的：探讨大豆蛋白与大豆低聚肽对负氮平衡老年小鼠在金黄色葡萄球菌表皮创伤感染下的免疫调节及炎症抑制的影响。方法：实验设空白组（正氮平衡）、模型组（负氮平衡）、蛋白组（负氮平衡灌胃大豆蛋白）、低聚肽组（负氮平衡灌胃大豆低聚肽），其中，蛋白组与低聚肽组灌胃液体总含氮量相等，以体质量计为650 mg/kg。经过背部表皮创伤感染金黄色葡萄球菌后灌胃，观察大豆蛋白与大豆低聚肽对小鼠体液免疫水平、炎症抑制水平、股直肌质量等的影响。结果：低蛋白饲料喂养能显著降低小鼠体质量、氮摄入量及血清总蛋白质量浓度（P＜0.05），大豆蛋白与大豆低聚肽能显著提高小鼠血清免疫球蛋白（immunoglobulin，Ig）M、 IgG及IgA质量浓度（P＜0.05），显著降低巨噬细胞炎性、RANTES质量浓度（P＜0.05），可降低炎症因子白细胞介素（inter leukin，IL）-1β、IL-6和TNF-α质量浓度，差异不显著，能显著提高股直肌湿质量与体质量比（P＜0.05），大豆低聚肽与大豆蛋白相比，可显著提高IgG质量浓度，显著降低蛋白-2质量浓度。结论：大豆蛋白与大豆低聚肽对体液免疫功能有显著的提高作用，对炎症反应有一定抑制作用，对肌肉萎缩有一定恢复作用，大豆低聚肽较大豆蛋白对体液免疫功能的提高以及早期炎症抑制作用更加显著。

关键词: 大豆蛋白, 大豆低聚肽, 负氮平衡, 金黄色葡萄球菌, 免疫调节, 炎症

Abstract: Objective: To investigate immune modulation and inflammatory inhibition of soybean protein and soybean oligopeptides on Staphylococcus aureus-induced epidermal trauma in aged mice with negative nitrogen balance. Methods: Mice were divided into four groups: blank control group (positive nitrogen balance), model group (negative nitrogen balance), soybean protein group (administration with soybean protein plus negative nitrogen balance), soybean oligopeptides group (administration with soybean oligopeptides plus negative nitrogen balance), among them, the total nitrogen content of the liquid in the soybean protein group and the soybean oligopeptides group was equal, calculated as body mass of 650 mg/kg. The mice were orally administered after back epidermal wound infection with Staphylococcus aureus. The effects of soybean protein and soybean oligopeptides on humoral immunity level, inflammation inhibition and rectus muscle mass were investigated. Results: Low protein diet significantly reduced body mass, nitrogen intake and total serum protein in mice (P < 0.05). Soybean protein and soybean oligopeptides significantly increased the serum levels of immunoglobulin (Ig) M, IgG and IgA (P < 0.05), and significantly reduced the contents of macrophage inflammatory protein-2 (MIP-2) and RANTES (P < 0.05), decreased the contents of interleukin (IL)-1β, IL-6 and TNF-α but without any significant difference, and significantly augmented the ratio of rectus femoris to body mass (P < 0.05). Compared with soybean protein, soybean oligopeptides significantly increased serum IgG level, and significantly reduced MIP-2 level (P < 0.05). Conclusion: Soybean protein and soybean oligopeptides can significantly enhance humoral immune function, inhibit inflammatory response and restore muscle atrophy. Soybean oligopeptides can enhance humoral immune function and inhibit early inflammatory response more significantly than soy protein.

Key words: soybean protein, soybean oligopeptides, negative nitrogen balance, Staphylococcus aureus, immunomodulatory effect, inflammation

中图分类号:

R151.1

张健，李雯晖，赵博雅，李赫，刘新旗，肖林. 大豆蛋白与大豆低聚肽对负氮平衡老年小鼠表皮创伤感染下的免疫调节作用[J]. 食品科学, 2018, 39(17): 145-151.

ZHANG Jian, LI Wenhui, ZHAO Boya, LI He, LIU Xinqi, XIAO Lin. Immune Modulation of Soybean Protein and Soybean Oligopeptides on Epidermal Trauma Infection in Aged Mice with Negative Nitrogen Balance[J]. FOOD SCIENCE, 2018, 39(17): 145-151.

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大豆蛋白与大豆低聚肽对负氮平衡老年小鼠表皮创伤感染下的免疫调节作用

Immune Modulation of Soybean Protein and Soybean Oligopeptides on Epidermal Trauma Infection in Aged Mice with Negative Nitrogen Balance

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