关键词: 人参发根, 抗氧化, 黑色素瘤, 细胞凋亡, 细胞周期

Abstract: The antioxidant capacity of Panax ginseng hairy root culture homogenate was measured by Cu2+ chelating ability, polyphenol oxidase activity and total antioxidant capacity assays, and its antimelanoma activity was evaluated by determining the cell apoptosis and cycle in melanoma cells A375 using flow cytometry. The results showed that the ginseng hairy root culture had Cu2+ chelating ability in a concentration-dependent manner, which was not significantly different from that of VC at a concentration of 100 mg/mL (P > 0.05). No significant difference in polyphenol oxidase activity was found between the presence of the homogenate and VC at 40, 60, 80 and 100 mg/mL (P > 0.05). The total antioxidant capacity of the homogenate was concentration dependent, 0.877 4 mmol FeSO4/g at 100 mg/mL. Compared with the control group, the ginseng hairy root culture significantly increased the cell apoptosis rate (P < 0.05), improved the percentage of cells in the S and G2/M phases and decreased the percentage of cells in the G0/G1 phase. These findings led to the conclusion that Panax ginseng hairy root has antioxidant and antimelanoma effects.

Key words: ginseng hairy root, antioxidant activity, melanoma, cell apoptosis, cell cycle