蓝靛果花色苷对高脂血症大鼠肝脏内与胆固醇代谢相关基因的作用

doi:10.7506/spkx1002-6630-201817028

食品科学 ›› 2018, Vol. 39 ›› Issue (17): 171-176.doi: 10.7506/spkx1002-6630-201817028

蓝靛果花色苷对高脂血症大鼠肝脏内与胆固醇代谢相关基因的作用

于伟1,2，张桂芳2，甄井龙3，宋雪建3，迟晓星3，王振宇4,*

1.东北林业大学林学院，黑龙江哈尔滨 150040；2.黑龙江八一农垦大学国家杂粮工程技术研究中心，黑龙江大庆 163319；3.黑龙江八一农垦大学食品学院，黑龙江大庆 163319；4.哈尔滨工业大学食品科学与工程学院，黑龙江哈尔滨 150001

出版日期:2018-09-15 发布日期:2018-09-18
基金资助:
黑龙江省教育厅科学技术研究项目（12541600）

Effect of Lonicera caerulea Anthocyan on the Role of Cholesterol Metabolism Related Genes in the Liver of Hyperlipidemic Rats

YU Wei1,2, ZHANG Guifang2, ZHEN Jinglong3, SONG Xuejian3, CHI Xiaoxing3, WANG Zhenyu4,*

1. School of Forestry, Northeast Forestry University, Harbin 150040, China; 2. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 4. College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150001, China

Online:2018-09-15 Published:2018-09-18

摘要/Abstract

摘要： 目的：研究蓝靛果花色苷对高脂血症大鼠肝脏肝X受体（liver X receptor α，LXRα）、B族I型清道夫受体（scavenger receptor class B, type I，SR-BI）、三磷酸腺苷结合盒转运蛋白G5（ATP-binding cassette transporter G5，ABCG5）、过氧化物酶体增殖物激活受体（peroxisome proliferator-activated receptor γ，PPARγ）及胆固醇7α-羟化酶（cholesterol 7α-hydroxylase，CYP7a1）基因表达的影响。方法：选择2 月龄雄性Wistar大鼠60 只，将大鼠随机分为 6 组，其中10 只给予普通饲料，其余50 只给予高脂饲料。30 d造模成功后，分别建立基础饲料正常对照组（ND，灌胃1.2 g/（kg·d）生理盐水）、高脂模型对照组（HFD，灌胃1.2 g/（kg·d）生理盐水）、阳性对照组（灌胃10 mg/（kg·d）辛伐他汀片）和蓝靛果花色苷低（HFD＋L）、中（HFD＋M）、高（HFD＋H）剂量组（分别灌胃4.0、40.0、120.0 mg/（kg·d）蓝靛果花色苷），持续28 d。利用实时荧光定量聚合酶链式反应检测大鼠肝脏组织中LXRα、SR-BI、ABCG5、PPARγ及CYP7a1 mRNA的表达情况。结果：经蓝靛果花色苷干预后，与HFD组相比：蓝靛果花色苷各剂量组肝脏中SR-BI、ABCG5 mRNA表达几乎不受影响，且无显著性差异（P＞0.05）；HFD＋M、 HFD＋H组LXRα、CYP7a1 mRNA表达升高且差异极显著（P＜0.01）；HFD＋L、HFD＋M、HFD＋H组 PPARγ mRNA表达降低且差异极显著（P＜0.01）。结论：蓝靛果花色苷通过提高高脂血症大鼠肝脏内LXRα、 CYP7a1 mRNA表达，降低PPARγ mRNA表达来调节高脂血症大鼠的血脂水平，预防动脉硬化的发生。关键词：蓝靛果；蓝靛果花色苷；高脂血症大鼠；肝X受体；B族I型清道夫受体；三磷酸腺苷结合盒转运蛋白G5；过氧化物酶体增殖物激活受体γ；胆固醇7α-羟化酶

关键词: 蓝靛果, 蓝靛果花色苷, 高脂血症大鼠, 肝X受体, B族I型清道夫受体, 三磷酸腺苷结合盒转运蛋白G5, 过氧化物酶体增殖物激活受体γ, 胆固醇7α-羟化酶

Abstract: Objective: The effect of Lonicera caerulea anthocyanin on the expression of liver X receptor α (LXRα), scavenger receptor class B, type I (SR-BI), ATP-binding cassette transporter G5 (ABCG5), peroxisome proliferator-activated receptor γ (PPARγ) and cholesterol 7α-hydroxylase (CYP7a1) in hyperlipidemic rat liver were investigated in this study. Methods: Totally 60 male Wistar rats at the age of two months were randomly divided into 6 groups including 10 rats with normal feed and 50 rats with high-fat diet for the diabetic model establishment. After model establishment in 30 days, the diabetic rats were divided 5 groups with basic diet group (ND, 1.2 g/(kg·d) physiological saline intragastric administration), high-fat diet group (HFD, 1.2 g/(kg·d) physiological saline intragastric administration), 10 mg/(kg·d) simvastatin tablet intragastric administration group as the positive control, and low dose, middle dose and high dose of Lonicera caerulea anthocyanin groups (HFD + L, HFD + M, HFD + H, Lonicera caerulea anthocyanin at 4.0, 40.0 and 120.0 mg/(kg·d). The intragastric administration was last for 28 days. The expression levels of LXRα, SB-BI, ABCG5, PPARγ and CYP7a1 were detected by quantitative real-time polymerase chain reaction (PCR). Results: Compared with HFD group, the mRNA expression levels of SR-BI and ABCG5 mRNA were not significant after the intervention of L. caerulea anthocyanins (P > 0.05); the mRNA expression levels of LXRα and CYP7a1 of HFD + M and HFD + H group in liver tissue were increased (P < 0.05 or P < 0.01); the mRNA expression levels of PPARγ of HFD + L, HFD + M and HFD + H group in liver tissue were decreased. Conclusion: The L. caerulea anthocyan can improve LXRα and CYP7a1 and can decrease PPARγ in liver tissue of hyperlipidemic rats; and regulate blood lipid level of hyperlipidemic rats, thereby preventing arteriosclerosis.

Key words: Lonicera caerulea, Lonicera caerulea anthocyan, hyperlipidemic rats, liver X receptor α, scavenger receptor class B, type I, ATP-binding cassette transporter G5, peroxisome proliferator-activated receptor γ, cholesterol 7α-hydroxylase

中图分类号:

TS201.4

于伟，张桂芳，甄井龙，宋雪建，迟晓星，王振宇. 蓝靛果花色苷对高脂血症大鼠肝脏内与胆固醇代谢相关基因的作用[J]. 食品科学, 2018, 39(17): 171-176.

YU Wei, ZHANG Guifang, ZHEN Jinglong, SONG Xuejian, CHI Xiaoxing, WANG Zhenyu. Effect of Lonicera caerulea Anthocyan on the Role of Cholesterol Metabolism Related Genes in the Liver of Hyperlipidemic Rats[J]. FOOD SCIENCE, 2018, 39(17): 171-176.

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蓝靛果花色苷对高脂血症大鼠肝脏内与胆固醇代谢相关基因的作用

Effect of Lonicera caerulea Anthocyan on the Role of Cholesterol Metabolism Related Genes in the Liver of Hyperlipidemic Rats

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