食品科学 ›› 2019, Vol. 40 ›› Issue (11): 195-200.doi: 10.7506/spkx1002-6630-20180604-030

• 包装贮运 • 上一篇    下一篇

O2/CO2气调下西兰花能量代谢与贮藏品质的关系

王 亮,陈 勇,郭衍银*,员丽苹,刘莎莎,张玉笑,张新华   

  1. 山东理工大学农业工程与食品科学学院,山东 淄博 255049
  • 出版日期:2019-06-15 发布日期:2019-06-28
  • 基金资助:
    国家自然科学基金面上项目(31671900);山东理工大学青年教师发展支持计划项目(4072-115008)

Relationship of Energy Metabolism and Storage Quality of Broccoli Head under O2/CO2 Controlled Atmospheres

WANG Liang, CHEN Yong, GUO Yanyin*, YUAN Liping, LIU Shasha, ZHANG Yuxiao, ZHANG Xinhua   

  1. School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, China
  • Online:2019-06-15 Published:2019-06-28

摘要: 能量在果蔬保鲜及衰老过程中起着至关重要的作用,为研究O2/CO2气调对西兰花能量代谢及保鲜效果的影响,本实验以自然空气为对照(CK),研究体积分数20% O2+80% CO2、50% O2+50% CO2、80% O2+20% CO2气调环境对西兰花10 ℃贮藏过程中pH值和丙酮酸脱氢酶(pyruvate dehydrogenase,PDH)、琥珀酸脱氢酶(succinate dehydrogenase,SDH)和细胞色素c氧化酶(cytochrome c oxidase,CCO)活力及能荷水平、色调(H值)、VC含量和水分质量分数的影响。结果表明:与50% O2+50% CO2处理组相比,过高比例的CO2(20% O2+80% CO2)处理可引起西兰花pH值的明显降低,进而造成PDH、SDH和CCO活力的下降和能荷水平的降低,最终造成贮藏期间西兰花VC含量、色调和水分质量分数的下降;过高比例的O2(80% O2+20% CO2)处理虽在贮藏前期提高了PDH、SDH和CCO活力,但在后期迅速下降,不利于建立平稳而持续的能量供应体系。适宜的O2/CO2气调如50%O2+50% CO2则可调节西兰花体内pH值和呼吸关键酶活力,建立持续稳定的能量供应体系并很好地维持西兰花贮藏品质。相关性分析表明,能荷水平与西兰花VC含量、色调和水分质量分数具有很高的正相关性,相关系数最高达0.996,因此,能荷变化可作为判断西兰花贮藏条件优劣的衡量指标。

关键词: 西兰花, O2/CO2气调, 能量, 保鲜, 相关分析

Abstract: Energy plays an essential role in the postharvest senescence of fruits and vegetables. In order to study the effect of O2/CO2 controlled atmosphere packaging on the energy metabolism and storage quality of broccoli at 10 ℃, different controlled atmospheres: 20% O2 + 80% CO2, 50% O2 + 50% CO2, and 80% O2 + 20% CO2 were employed as the experimental groups while natural air was used as the control. pH, the activities of pyruvate dehydrogenase (PDH), succinate dehydrogenase (SDH) and cytochrome c oxidase (CCO), energy charge, ascorbic acid, color value (hue, H) and water content were periodically measured during storage. The results showed that compared with the 50% O2 + 50% CO2 treatment, excessive proportion of CO2 such as 20% O2 + 80% CO2 significantly decreased the pH, PDH, SDH and CCO activities, and energy charge, and finally resulted in a decrease of VC content, H value and water content in broccoli during storage. Excessive proportion of O2 such as 80% O2 + 20% CO2 increased the activities of PDH, SDH and CCO during the early storage period followed by a rapid drop during the later storage period, which hindered the establishment of a stable and continuous energy supply system. Appropriate controlled atmosphere with 50% O2 + 50% CO2 regulated the pH and respiratory key enzyme activity in broccoli during the whole storage period to establish a sustainable and stable energy supply system, and consequently ensure the quality maintenance of broccoli during storage. The correlation analysis showed a highly positive correlation between energy charge and VC, energy charge and H value, and energy charge and water content, with the highest correlation coefficient of 0.996. Therefore, energy charge can be used as an important indicator in measuring and optimizing the storage conditions for broccoli.

Key words: broccoli, O2/CO2 controlled atmospheres, energy, preservation, correlation analysis

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