食品科学 ›› 2019, Vol. 40 ›› Issue (19): 32-39.doi: 10.7506/spkx1002-6630-20181028-322

• 基础研究 • 上一篇    下一篇

环境因素对叶黄素纳米乳液稳定性及其抗氧化活性的影响

李季楠,胡浩,吴雪娇,吴艳   

  1. (上海交通大学农业与生物学院,上海 200240)
  • 出版日期:2019-10-15 发布日期:2019-10-25
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400205)

Effect of Environmental Conditions on the Stability and Free Radical Scavenging Capacity of Lutein-Enriched Nanoemulsion

LI Jinan, HU Hao, WU Xuejiao, WU Yan   

  1. (School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China)
  • Online:2019-10-15 Published:2019-10-25

摘要: 以酪蛋白酸钠为乳化剂,高压均质构建叶黄素纳米乳液载运体系,并评价其对1,1-二苯基-2-三硝基苯肼自由基和2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)阳离子自由基的清除能力和氧自由基吸收能力,及环境因素(pH值、热处理、离子强度和浓缩)对乳液体系中叶黄素稳定性和自由基清除活性的影响。结果表明:纳米乳液体系能够显著提高叶黄素的自由基清除活性(P<0.05);pH值为2.0或离子强度为50 mmol/L和100 mmol/L NaCl时,纳米乳液的叶黄素质量浓度和自由基清除活性有不同程度的减小,旋转蒸发浓缩后叶黄素质量浓度和自由基清除率均显著增加(P<0.05),而热处理(60、80 ℃和100 ℃)对叶黄素纳米乳液体系的影响则相对较小。

关键词: 叶黄素, 纳米乳液, 自由基清除活性, 稳定性

Abstract: A lutein-enriched nanoemulsion stabilized by sodium caseinate was constructed by high-pressure homogenization, and its 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging capacity and oxygen radical absorbance capacity (ORAC) were investigated. The effects of environmental conditions on lutein stability and free radical scavenging capacity in the nanoemulsion were evaluated. The free radical scavenging capacity of lutein was improved significantly by incorporation into the emulsion system (P < 0.05). In addition, the free radical scavenging activity and lutein content of the nanoemulsion decreased to varying degrees when exposed to pH 2.0 as well as 50 and 100 mmol/L NaCl, but increased significantly after condensation treatment (P < 0.05); in contrast, thermal treatment (60, 80, 100 ℃) had little impact on the two parameters.

Key words: lutein, nanoemulsion, free radical scavenging capacity, stability

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