顶空-固相微萃取法与水蒸气蒸馏法提取山银花挥发性组分的比较

doi:10.7506/spkx1002-6630-20181029-343

食品科学 ›› 2020, Vol. 41 ›› Issue (4): 178-184.doi: 10.7506/spkx1002-6630-20181029-343

顶空-固相微萃取法与水蒸气蒸馏法提取山银花挥发性组分的比较

张鹏云，管维，李蓉，龙春霞，卢俊文，林淑绵，张峰

（1.中山海关技术中心，广东中山 528400；2.洛阳市疾病预防控制中心，河南洛阳 471000；3.广东药科大学公共卫生学院，广东中山 528458；4.中国检验检疫科学研究院食品安全研究所，北京 100176）

出版日期:2020-02-25 发布日期:2020-03-02
基金资助:
“十三五”国家重点研发计划重点专项（2017YFF0211000）

Comparison of Volatile Components from Flos Lonicerae Extracted by Headspace Solid Phase Microextraction and Steam Distillation

ZHANG Pengyun, GUAN Wei, LI Rong, LONG Chunxia, LU Junwen, LIN Shumian, ZHANG Feng

(1. Technology Center, Zhongshan Customs, Zhongshan 528400, China; 2. Luoyang Center for Disease Control and Prevention, Luoyang 471000, China; 3. School of Public Health, Guangdong Pharmaceutical University, Zhongshan 528458, China; 4. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, China)

Online:2020-02-25 Published:2020-03-02

摘要/Abstract

摘要： 通过单因素试验和正交试验优化顶空-固相微萃取（headspace solid-phase microextraction，HS-SPME）法提取山银花挥发性成分的条件，并采用气相色谱-串联质谱仪和气相色谱-四极杆-飞行时间质谱仪分别对HS-SPME法和水蒸气蒸馏（steam distillation，SD）法的提取物进行分析。结果显示，HS-SPME法的优化条件为萃取温度60 ℃、平衡时间25 min、萃取时间60 min、解吸时间5 min，该条件下鉴定出59 种组分，占总量的97.17%，主要成分为萜烯类、醇类和醛类；SD法的提取物鉴定出68 种组分，占总量的91.69%，主要成分为醇类、醛类和酮类。两种方法提取的挥发油组分种类及含量差异较大。该研究为山银花的质量鉴定和开发利用提供了科学依据。

关键词: 山银花, 顶空固相微萃取法, 水蒸气蒸馏法, 挥发性成分

Abstract: The conditions for extracting volatile components from Flos Lonicerae by headspace solid-phase microextraction (HS-SPME) were optimized by one-factor-at-a-time and orthogonal array design methods. Gas chromatography-mass spectrometry (GC-MS) and gas chromatography-quadrupole time-of-flight mass spectrometry (GC-QTOF MS) were used to detect the extracts obtained by HS-SPME and steam distillation (SD). The results showed that the optimum conditions of HS-SPME were as follows: extraction temperature 60 ℃, equilibrium time 25 min, extraction time 60 min, and desorption time 5 min. Under these conditions, 59 components were identified, accounting for 97.17% of the total amount, and the main components extracted by HS-SPME were terpenes, alcohols and aldehydes; a total of 68 components were identified from the SD extract, accounting for 91.69% of the total amount, with alcohols, aldehydes and ketones being dominant. Accordingly, the types and contents of volatile oil components extracted by the two methods were quite different. This study provides a scientific basis for quality identification, development and utilization of Flos Lonicerae.

Key words: Flos Lonicerae, headspace solid-phase microextraction, steam distillation, volatile components

中图分类号:

R284.1

张鹏云，管维，李蓉，龙春霞，卢俊文，林淑绵，张峰. 顶空-固相微萃取法与水蒸气蒸馏法提取山银花挥发性组分的比较[J]. 食品科学, 2020, 41(4): 178-184.

ZHANG Pengyun, GUAN Wei, LI Rong, LONG Chunxia, LU Junwen, LIN Shumian, ZHANG Feng. Comparison of Volatile Components from Flos Lonicerae Extracted by Headspace Solid Phase Microextraction and Steam Distillation[J]. FOOD SCIENCE, 2020, 41(4): 178-184.

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顶空-固相微萃取法与水蒸气蒸馏法提取山银花挥发性组分的比较

Comparison of Volatile Components from Flos Lonicerae Extracted by Headspace Solid Phase Microextraction and Steam Distillation

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