桑叶生物碱对四氯化碳联合高脂饮食诱导的小鼠肝纤维化的改善作用

doi:10.7506/spkx1002-6630-20190314-181

食品科学 ›› 2020, Vol. 41 ›› Issue (7): 146-152.doi: 10.7506/spkx1002-6630-20190314-181

桑叶生物碱对四氯化碳联合高脂饮食诱导的小鼠肝纤维化的改善作用

王祖文，杨忠敏，黄先智，丁晓雯

（1.西南大学食品科学学院，食品科学与工程国家级实验教学示范中心，重庆市农产品加工及贮藏重点实验室，重庆 400716；2.西南大学科技处，重庆 400716）

出版日期:2020-04-15 发布日期:2020-04-20
基金资助:
现代农业产业技术体系建设专项（CARS-18）

Improvement Effect of Mulberry Leaf Alkaloids on Hepatic Fibrosis Induced by Carbon Tetrachloride Combined with High-Fat Diet in Mice

WANG Zuwen, YANG Zhongmin, HUANG Xianzhi, DING Xiaowen

(1. National Demonstration Center for Experimental Food Science and Technology Education, Chongqing Key Laboratory of Processing and Storage of Agricultural Products, College of Food Science, Southwest University, Chongqing 400716, China; 2. Science and Technology Department, Southwest University, Chongqing 400716, China)

Online:2020-04-15 Published:2020-04-20

摘要/Abstract

摘要： 目的：探讨桑叶生物碱对四氯化碳（CCl4）联合高脂饮食诱导的小鼠肝纤维化的改善作用，为桑叶的科学利用提供依据。方法：采用腹腔注射体积分数10% CCl4橄榄油溶液（5 mL/kg mb）联合高脂饮食，持续8 周，建立小鼠肝纤维化模型。造模结束后，小鼠灌胃低（50 mg/kg mb）、中（100 mg/kg mb）、高（200 mg/kg mb）剂量的桑叶生物碱及阳性药物水飞蓟宾（100 mg/kg mb），连续45 d。观察各组小鼠体质量、肝脏指数变化；测定小鼠血脂、血浆肝功能相关指标和肝纤维化标志物含量；苏木精-伊红染色观察小鼠肝组织病理学变化。结果：与模型组比较，阳性药物水飞蓟宾和桑叶生物碱各剂量组小鼠的肝脏指数、血浆谷丙转氨酶、谷草转氨酶、碱性磷酸酶活力，总胆红素、直接胆红素浓度，血浆胆固醇、甘油三酯含量，肝纤维化标志物透明质酸、层黏连蛋白、IV型胶原、III型前胶原质量浓度均有所下降；总蛋白、白蛋白质量浓度均显著上升（P＜0.05）；体质量虽有所增加，但与模型组差异不显著（P＞0.05）；此外，桑叶生物碱高剂量组与阳性药物组之间结果差异不显著（P＞0.05），但均未恢复至正常水平。肝脏病理组织切片图像显示，阳性药物组和桑叶生物碱各剂量组肝脏病变程度明显减轻。结论：桑叶生物碱对CCl4联合高脂饮食诱导的小鼠肝纤维化具有改善作用，且与水飞蓟宾的效果相似。

关键词: 桑叶生物碱, 四氯化碳, 高脂饮食, 肝纤维化, 改善作用

Abstract: Objective: To study the improvement effect of mulberry leaf alkaloids on hepatic fibrosis induced by carbon tetrachloride (CCl4) combined with a high-fat diet in mice. Methods: Sixty male Kunming mice were randomly divided into six groups, normal control, model, positive control (100 mg/kg mb of silibinin), and gavage administration of mulberry leaf alkaloids at low, medium and high doses (50, 100 and 200 mg/kg mb). A mouse model of hepatic fibrosis was established by intraperitoneal injection of 10% CCl4 in olive oil combined with feeding of a high-fat diet for 8 weeks. After administration for 45 days, body mass and liver index were observed, and blood lipids, liver function-related indicators and fibrosis markers in plasma were measured. The histopathological characteristics of liver tissues were observed by hematoxylin-eosin staining. Results: Compared with the model group, liver index, the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and the concentrations of total bilirubin (TBil) and direct bilirubin (DBil), total cholesterol (TC) and triglycerides (TG) and hepatic fibrosis markers such as hyaluronicacid (HA), laminin (LN), type IV collagen (IV-C) and type III precollagen (PC-III) in plasma were significantly decreased, and the levels of total protein (TP) and albumin (ALB) were significantly increased in the positive control group and the three treatment groups (P < 0.05). Body mass increased but not significantly in the above four groups (P > 0.05). Moreover, there was no significant difference between the high-dose group (200 mg/kg mb) and the positive drug group, and but neither restored the parameters investigated to normal levels. The histopathological examination showed that the degree of liver lesions in the positive control group and the three treatment groups was significantly reduced. Conclusion: Mulberry leaf alkaloids may improve hepatic fibrosis induced by CCl4 combined with high-fat diet as effectively as silibinin.

Key words: mulberry leaf alkaloids, carbon tetrachloride, high-fat diet, hepatic fibrosis, improvement effect

中图分类号:

R285.5

王祖文，杨忠敏，黄先智，丁晓雯. 桑叶生物碱对四氯化碳联合高脂饮食诱导的小鼠肝纤维化的改善作用[J]. 食品科学, 2020, 41(7): 146-152.

WANG Zuwen, YANG Zhongmin, HUANG Xianzhi, DING Xiaowen. Improvement Effect of Mulberry Leaf Alkaloids on Hepatic Fibrosis Induced by Carbon Tetrachloride Combined with High-Fat Diet in Mice[J]. FOOD SCIENCE, 2020, 41(7): 146-152.

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桑叶生物碱对四氯化碳联合高脂饮食诱导的小鼠肝纤维化的改善作用

Improvement Effect of Mulberry Leaf Alkaloids on Hepatic Fibrosis Induced by Carbon Tetrachloride Combined with High-Fat Diet in Mice

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