芋艿采后主要病原菌分离鉴定及桧木醇抑菌效果

doi:10.7506/spkx1002-6630-20190521-248

食品科学 ›› 2020, Vol. 41 ›› Issue (12): 136-142.doi: 10.7506/spkx1002-6630-20190521-248

芋艿采后主要病原菌分离鉴定及桧木醇抑菌效果

赵璐藐，郜海燕，刘瑞玲，丁玉庭，韩延超，陈杭君

（1.浙江工业大学食品科学与工程学院，浙江杭州 310014；2.浙江省农业科学院食品科学研究所，农业农村部果品产后处理重点实验室，中国轻工业果蔬保鲜与加工重点实验室，浙江省果蔬保鲜与加工技术研究重点实验室，浙江杭州 310021）

出版日期:2020-06-25 发布日期:2020-06-22
基金资助:
现代农业产业技术体系建设专项（CARS-24-E-01）；“十三五”国家重点研发计划重点专项（2018YFD0401304）；浙江省万人计划人才培养项目（2018R52020）；浙江省重点研发计划项目（2019C02079）；宁波工程学院奉化研究院科学基金项目（FHI-018113）

Identification of Dominant Postharvest Pathogens of Taro and Antifungal Activity of Hinokitiol against Them

ZHAO Lumiao, GAO Haiyan, LIU Ruiling, DING Yuting, HAN Yanchao, CHEN Hangjun

(1. College of Food Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, China; 2. Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Hangzhou 310021, China)

Online:2020-06-25 Published:2020-06-22

摘要/Abstract

摘要： 为明确芋艿采后腐烂的主要病原菌种类，研究桧木醇对病原菌的抑制效果，为采后病害防治提供依据。本实验通过组织分离法，从采后自然发病的奉化芋艿上分离纯化主要病原真菌，基于科赫氏法则，进行病原菌致病性检测，并通过形态观察和真菌rDNA-ITS序列分析对病原菌进行鉴定；在此基础上进一步探讨桧木醇对病原菌的抑制效果，研究桧木醇对病原菌菌丝生长、孢子萌发、丙二醛浓度、亚细胞结构形态的影响。结果表明，贮藏过程中引起芋艿腐烂变质的主要病原菌经鉴定为镰刀菌属（Fusarium sp.）和可可毛色二孢菌（Lasiodiplodia theobromae）；桧木醇处理可抑制此两种病原菌菌丝生长和孢子萌发，加剧菌丝膜脂过氧化，并破坏菌丝体亚细胞结构，抑菌效果明显，在控制芋艿采后病害方面具有较好的应用前景。

关键词: 芋艿, 采后病原菌, 分离鉴定, 桧木醇, 抑菌效果

Abstract: To provide evidence for the control of taro postharvest diseases, the main pathogens causing postharvest decay of taro and the antifungal effect of hinokitiol against these pathogens were investigated. In this study, the pathogenic fungal strains were isolated from naturally decaying taro grown in Fenghua, Zhejiang province by tissue separation method and identified by morphological characteristics and rDNA-ITS sequence analysis, and their pathogenicity was evaluated by Koch’s postulate. The antifungal activity of hinokitiol was investigated by evaluating its effects on the mycelial growth, spore germination, malondialdehyde content and ultrastructure of the dominant pathogens. The results showed that the two pathogenic strains isolated were identified as Fusarium sp. and Lasiodiplodia theobromae respectively. Hinokitiol exhibited obvious antifungal activity against the pathogens. It inhibited mycelial growth and spore germination, exacerbated membrane lipid peroxidation, and destroyed the subcellular structure of mycelia. Accordingly, this study indicated that hinokitiol can be a promising candidate for the control of postharvest diseases of taro.

Key words: taro, postharvest pathogen, isolation and identification, hinokitiol, antifungal effect

中图分类号:

S379.9

赵璐藐，郜海燕，刘瑞玲，丁玉庭，韩延超，陈杭君. 芋艿采后主要病原菌分离鉴定及桧木醇抑菌效果[J]. 食品科学, 2020, 41(12): 136-142.

ZHAO Lumiao, GAO Haiyan, LIU Ruiling, DING Yuting, HAN Yanchao, CHEN Hangjun. Identification of Dominant Postharvest Pathogens of Taro and Antifungal Activity of Hinokitiol against Them[J]. FOOD SCIENCE, 2020, 41(12): 136-142.

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芋艿采后主要病原菌分离鉴定及桧木醇抑菌效果

Identification of Dominant Postharvest Pathogens of Taro and Antifungal Activity of Hinokitiol against Them

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