库车小白杏内生细菌多样性及其产酶特性

doi:10.7506/spkx1002-6630-20190529-359

食品科学 ›› 2020, Vol. 41 ›› Issue (14): 203-209.doi: 10.7506/spkx1002-6630-20190529-359

库车小白杏内生细菌多样性及其产酶特性

刘晓静，楚敏，朱静，顾美英，唐琦勇，孙建，朱璇，张志东,

（1.新疆农业大学食品科学与药学学院，新疆乌鲁木齐 830052；2.新疆农业科学院微生物应用研究所，新疆特殊环境微生物实验室，新疆乌鲁木齐 830091）

发布日期:2020-07-29
基金资助:
新疆维吾尔自治区自然科学基金项目（2018D01A15）

Diversity and Functional Enzymes of Endophytic Bacteria in Kuqa Apricot

LIU Xiaojing, CHU Min, ZHU Jing, GU Meiying, TANG Qiyong, SUN Jian, ZHU Xuan, ZHANG Zhidong

(1. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, China; 2. Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, ürümqi 830091, China)

Published:2020-07-29

摘要/Abstract

摘要： 分析采后库车小白杏果实内生细菌多样性，挖掘潜在的微生物资源及功能。利用高通量测序法对采后库车小白杏内生细菌结构组成进行分析，同时，采用传统分离培养法对内生细菌进行分离筛选，并对所获得的菌株进行16S rRNA基因序列分子鉴定，进而对相关菌株的产酶特性进行分析。高通量测序结果显示，采后库车小白杏内生细菌共包括406 个操作分类单元，涉及9 个门，94 个属。其中，拟杆菌属（Bacteroidetes）、芽孢杆菌属（Bacillus）、类芽孢杆菌属（Paenibacillus）和未分类菌群等物种多样性最为丰富。通过分离筛选共获得99 株菌，分别归属于放线菌门（Actinobacteria）、变形杆菌门（Proteobacteria）、厚壁菌门（Firmicutes）、拟杆菌门（Bacteroidetes）4 个菌门的28 个属。同时，获得7 个未在高通量测序结果中检出的属类群，发现潜在新种5 个。实验获得了一批蛋白酶、脂肪酶、淀粉酶、纤维素酶和果胶酶产生菌。本研究的开展为深度挖掘库车小白杏果实中的潜在微生物资源及相关酶制剂的筛选和研制提供丰富的材料，同时，也为从微生物角度揭示小白杏腐败变质等研究提供新思路。

关键词: 库车小白杏；内生细菌；多样性

Abstract: This study aimed to analyze the diversity of endophytic bacteria in postharvest Kuqa apricot for the purpose of discovering potential microbial resources and their functions. The composition of the endophytic bacterial community was analyzed by high-throughput sequencing. Meanwhile, endophytic bacteria were isolated using the traditional culture method, and identified by 16S rRNA gene sequence analysis. The enzyme activities of the isolates were tested. High-throughput sequencing results showed that 406 operational taxonomic units (OTUs) affiliated to 94 genera of 9 phyla were observed. Bacteroidetes, Bacillus, Paenibacillus and unclassified bacteria were the most abundant species. A total of 99 isolates were obtained, which were classified into 28 genera in the phyla of Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes. In addition, seven genera not detected by high-throughput sequencing and 5 potential novel species were discovered. At the same time, several isolates were found to be capable of producing protease, lipase, cellulase, amylase and pectase, which could provide abundant materials for further mining and development of potential microbial resource and enzymes from Kuqa apricot. The results provide a new idea for studying microbial deterioration of Kuqa apricot.

Key words: Kuqa apricot; endophytic bacteria; diversity

中图分类号:

Q939.1

刘晓静，楚敏，朱静，顾美英，唐琦勇，孙建，朱璇，张志东,. 库车小白杏内生细菌多样性及其产酶特性[J]. 食品科学, 2020, 41(14): 203-209.

LIU Xiaojing, CHU Min, ZHU Jing, GU Meiying, TANG Qiyong, SUN Jian, ZHU Xuan, ZHANG Zhidong. Diversity and Functional Enzymes of Endophytic Bacteria in Kuqa Apricot[J]. FOOD SCIENCE, 2020, 41(14): 203-209.

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库车小白杏内生细菌多样性及其产酶特性

Diversity and Functional Enzymes of Endophytic Bacteria in Kuqa Apricot

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