食品科学 ›› 2020, Vol. 41 ›› Issue (15): 48-53.doi: 10.7506/spkx1002-6630-20190725-338

• 基础研究 • 上一篇    下一篇

二氢杨梅素-Ag+纳米乳液的制备及其对金黄色葡萄球菌抑制性能与机理

丁利君,黄梓浩,刘丹   

  1. (广东工业大学轻工化工学院,广东 广州 510006)
  • 出版日期:2020-08-15 发布日期:2020-08-19
  • 基金资助:
    国家自然科学基金青年科学基金项目(21706037)

Preparation of Dihydromyricetin-Ag+ Nanoemulsion and Its Inhibitory Effect and Mechanism on Staphylococcus aureus

DING Lijun, HUANG Zihao, LIU Dan   

  1. (College of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China)
  • Online:2020-08-15 Published:2020-08-19

摘要: 为解决二氢杨梅素(dihydromyricetin,DMY)水难溶性和不稳定的问题,构建DMY-Ag+纳米乳液体系,通过协同作用提高体系的抑菌性能,并对纳米乳液抑菌机理进行初探。结果表明,DMY对金黄色葡萄球菌的最低抑菌浓度为1.25 mg/mL;通过DMY-Ag+纳米乳液的表征结果,确认其水包油的结构,其水溶液稳定;通过全自动生长曲线、抑菌圈直径及平板计数的方法研究该纳米乳液对金黄色葡萄球菌的抑菌性能,相对于单种抑菌处理液(DMY或Ag+),DMY-Ag+纳米乳液达到了较好的协同抑制效果;对该纳米乳液的抑菌机理研究结果表明,纳米乳液通过破坏细胞壁膜的完整性和选择透过性,使得胞内大小分子物质流向细胞外,并使细胞呼吸代谢循环链中的脱氢酶活力下降或丧失,从而影响细胞正常的物质和能量需要,最终达到抑制或杀死细菌的目的。本实验可为DMY-Ag+纳米乳液作为一种天然高效的抑菌剂应用于食品行业提供理论参考,使其具有更高的开发价值。

关键词: 二氢杨梅素, 银离子, 纳米乳液, 金黄色葡萄球菌, 抑菌机理

Abstract: In order to overcome the poor water solubility and instability of dihydromyricetin (DMY), a dihydromyricetin-silver ion (DMY-Ag+) nanoemulsion system was constructed, which could exert strong synergistic antibacterial activity. Its antibacterial mechanism was also investigated. The results showed that the minimum inhibitory concentration (MIC) of DMY against Staphylococcus aureus was 1.25 mg/mL. The DMY-Ag+ nanoemulsion was characterized as an oil-in-water emulsion. Its antibacterial activity was evaluated by the bacterial growth curve, the inhibition zone diameter and plate count. It was found that the nanoemulsion had an obvious synergistic effect when compared with DMY or Ag+ alone. The nanoemulsion could damage the integrity and selective permeability of the cell membrane, resulting in leakage of intracellular components, and partially or completely inhibit dehydrogenase activity in the cellular respiratory chain, thereby affecting normal energy supply and ultimately inhibiting or killing the bacterial cells. This study highlights the potential of the DMY-Ag+ nanoemulsion as a natural and highly effective bacteriostatic agent in the food industry.

Key words: dihydromyricetin, silver ion, nanoemulsion, Staphylococcus aureus, bacteriostatic mechanism

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