包装充氧量对无水活运花鲈鳃组织结构及相关酶活性的影响

doi:10.7506/spkx1002-6630-20190129-380

食品科学 ›› 2020, Vol. 41 ›› Issue (15): 269-274.doi: 10.7506/spkx1002-6630-20190129-380

包装充氧量对无水活运花鲈鳃组织结构及相关酶活性的影响

张玉晗，谢晶

（上海海洋大学食品学院，上海水产品加工及贮藏工程技术研究中心，上海冷链装备性能与节能评价专业技术服务平台，食品科学与工程国家级实验教学示范中心（上海海洋大学），上海 201306）

出版日期:2020-08-15 发布日期:2020-08-19
基金资助:
现代农业产业技术体系建设专项（CARS-47）；2019年上海市科技兴农重点攻关项目（2019-02-08-00-10-F01143）；上海市科委平台能力建设项目（19DZ2284000）；上海市科委公共服务平台建设项目（17DZ2293400）

Effect of Packaging Oxygen Concentration on Gill Structure and Related Enzyme Activities in Lateolabrax maculatus during Waterless Live Transportation

ZHANG Yuhan, XIE Jing

(Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center, Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, National Experimental Teaching Demonstration Center for Food Science and Engineering (Shanghai Ocean University), College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China)

Online:2020-08-15 Published:2020-08-19

摘要/Abstract

摘要： 为缩小无水保活运输包装充氧量的范围，降低运输包装环境成本。设定不同充氧量（O2体积分数分别为60%、80%、98%）包装花鲈，冷却后进行无水活运，检测其血清丙二醛（malondialdehyde，MDA）、超氧化物歧化酶（superoxide dismutase，SOD）、体表黏液溶菌酶活力，并观察鳃组织形态学的变化。结果表明：在相同运输时间下，60% O2包装组花鲈血清中MDA浓度最低，80% O2包装组花鲈血清中SOD活力保持稳定，运输中98% O2包装组花鲈体表黏液溶菌酶活力处于最低水平。取正常花鲈鱼鳃（CK）、运输0 h（降温未包装运输）和运输8 h（O2体积分数60%、80%、98%）的鳃丝进行组织切片观察：运输0 h时鳃丝明显肿胀，运输8 h时鳃小片均出现融合黏连；鳃小片上皮细胞包括环形微嵴（circulars microridged，CM）、星点棒状（star point rod-shaped，SPRS）和微绒毛（microvilli，MIC），60%、80%、98% O2包装组运输8 h时，鳃小片表面因无水操作造成皱起不平，CM、SPRS数量减少，MIC几乎不可见；鳃丝内部游离线粒体增多且趋于表皮，黏液细胞减少，表皮边缘CM变粗或脱落，80% O2包装组鱼鳃组织形态保存完好，更接近CK组。因此，建议无水活运包装充氧体积分数选用60%～80%。

关键词: 花鲈, 包装充氧量, 鳃组织, 无水活运, 溶菌酶

Abstract: In the present study, we attempted to narrow the oxygen concentration range in the packaging atmosphere during waterless live fish transportation and hence reduce the packaging cost. Lateolabrax maculates were packaging with various oxygen concentrations (60%, 80% and 98%) and cooled before waterless live transportation for up to 8 h. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in serum, the activity of body surface mucus lysozyme (LZM) and the morphology of the gill were determined during the transportation period. Results indicated that at each transportation time, the lowest serum MDA level occurred in the 60% O2 group. Serum SOD activity in the 80% O2 group remained stable during the 8 h transportation period, and LZM activity was always at the lowest level in the 98% O2 group. The gill filaments were swollen at 0 h, and the gill lamellas were fused and adhered together at 8 h; circular microridges (CM), star point rod-shaped (SPRS) structures and microvilli (MIC) were observed on the surface of gill lamellar epithelial cells under microscopy. At 8 h, in all three groups, the surface of gill lamellas was wrinkled and rough due to lack of water, the number of CM and SPRS declined and MIC almost disappeared. Moreover, free mitochondria inside the gill filaments increased and moved to the epidermis, mucous cells decreased, and CM at the edge of the epidermis became thicker or were exfoliated. In the 80% O2 group, the gill morphology was intact, more similar to that of the control group. Therefore, packaging with oxygen concentration of 60%–80% could be recommended for waterless live transportation of Lateolabrax maculatus.

Key words: Lateolabrax maculatus, packaging oxygen concentration, fish gill, waterless live transportation, lysozyme

中图分类号:

TS254.4

张玉晗, 谢晶. 包装充氧量对无水活运花鲈鳃组织结构及相关酶活性的影响[J]. 食品科学, 2020, 41(15): 269-274.

ZHANG Yuhan, XIE Jing. Effect of Packaging Oxygen Concentration on Gill Structure and Related Enzyme Activities in Lateolabrax maculatus during Waterless Live Transportation[J]. FOOD SCIENCE, 2020, 41(15): 269-274.

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包装充氧量对无水活运花鲈鳃组织结构及相关酶活性的影响

Effect of Packaging Oxygen Concentration on Gill Structure and Related Enzyme Activities in Lateolabrax maculatus during Waterless Live Transportation

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