食品科学 ›› 2020, Vol. 41 ›› Issue (17): 133-138.doi: 10.7506/spkx1002-6630-20190810-113

• 营养卫生 • 上一篇    下一篇

盐胁迫环境下发菜胞外多糖抗氧化作用及镇痛抗炎活性

常相娜,陈雪峰,龚频,杨文娟,王兰,袁霞,刘宁   

  1. (陕西科技大学食品与生物工程学院,陕西 西安 710021)
  • 出版日期:2020-09-15 发布日期:2020-09-16
  • 基金资助:
    国家自然科学基金青年科学基金项目(81803698); 陕西省重点研发计划项目(2017NY-146;2017NY-103;2018ZDXM-NY-054)

Antioxidant, Analgesic and Anti-inflammatory Activities of Extracellular Polysaccharide of Nostoc flagelliforme under Salt Stress

CHANG Xiangna, CHEN Xuefeng, GONG Pin, YANG Wenjuan, WANG Lan, YUAN Xia, LIU Ning   

  1. (School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China)
  • Online:2020-09-15 Published:2020-09-16

摘要: 为研究NaCl(0.3 mol/L)胁迫条件下发菜胞外多糖体外抗氧化作用与体内镇痛抗炎活性,本实验通过体外自由基(羟自由基、O2-·、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical,DPPH)自由基)清除能力实验评价盐胁迫发菜胞外多糖的抗氧化作用;采取小鼠温浴甩尾法镇痛实验并结合皮下注射及腹腔注射两种给药方式分析盐胁迫发菜胞外多糖的镇痛作用;通过观察二甲苯致小鼠耳廓肿胀的抑制效果分析盐胁迫发菜胞外多糖的抗炎作用。结果表明:相比于无盐胁迫的发菜胞外多糖,盐胁迫发菜胞外多糖对羟自由基、O2-·、DPPH自由基3 种不同类型的自由基清除能力均有提高;盐胁迫发菜胞外多糖镇痛活性优于无盐胁迫发菜胞外多糖,痛阈值显著提高(P<0.05),腹腔注射比皮下注射多糖吸收速率更快,腹腔注射后20 min药效达到峰值,皮下注射后30 min药效达到峰值;盐胁迫发菜胞外多糖有更好的抗炎效果,且盐胁迫发菜胞外多糖的抗炎作用呈现出一定的量效关系,高剂量(30 mg/kg mb)对耳肿胀治疗效果最佳。综上所述,相较于无盐胁迫发菜胞外多糖,盐胁迫发菜胞外多糖具有更好的体外抗氧化与抗炎镇痛活性。

关键词: 发菜;胞外多糖;盐胁迫;抗氧化活性;镇痛活性;抗炎活性

Abstract: The antioxidant, analgesic and anti-inflammatory activities of the extracellular polysaccharide from Nostoc flagelliforme cultured in a liquid medium with 0.3 mol/L NaCl were studied. The in vitro antioxidant activity was investigated by determining the scavenging capacity against hydroxyl radical (·OH), superoxide anion radical (O2-·) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The mouse warm-water tail-flick test was used to study the analgesic effect of subcutaneous versus intraperitoneal administration of the extracellular polysaccharide. The anti-inflammatory effect was assessed by observing the inhibition of dimethylbenzene-induced ear edema in mice. The results showed that the scavenging capacities against ·OH, O2-· and DPPH radical of the extracellular polysaccharide produced under salt stress were higher than those in the absence of salt stress. In addition, the former had a stronger analgesic activity than did the latter with a significant higher pain threshold (P < 0.05). The extracellular polysaccharide was absorbed faster when administered subcutaneously?than when administered?intraperitoneally, and the efficacy of subcutaneous injection reached its peak at 30 min, whereas the efficacy of intraperitoneal injection reached its peak at 20 min. Moreover, the anti-inflammatory activity of the extracellular polysaccharide produced under salt stress was stronger and in a dose-dependent manner, and it at a high dose (30 mg/kg mb) showed the best anti-inflammatory effect.

Key words: Nostoc flagelliforme; extracellular polysaccharide; salt stress; antioxidant activity; analgesic activity; anti-inflammatory activity

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