食品科学 ›› 2020, Vol. 41 ›› Issue (20): 151-158.doi: 10.7506/spkx1002-6630-20190809-106

• 成分分析 • 上一篇    下一篇

菌种对发酵红茶水溶液香气品质的影响

林琦,李利君,伍菱,黄高凌,翁淑燚,倪辉,李清彪,张树文,黄幼林   

  1. (1.集美大学食品与生物工程学院,福建 厦门 361021;2.福建省食品微生物与酶工程重点实验室,福建 厦门 361021;3.厦门市食品生物工程技术研究中心,福建 厦门 361021;4.大闽食品(漳州)有限公司,福建 厦门 361021;5.集美大学理学院,福建 厦门 361021)
  • 出版日期:2020-10-25 发布日期:2020-10-23
  • 基金资助:
    国家自然科学基金面上项目(31871765);福建省自然科学基金项目(2017J01581)

Effects of Fermentation Strains on the Aroma Quality of Black Tea Infusion

LIN Qi, LI Lijun,, WU Ling,, HUANG Gaoling,, WENG Shuyi, NI Hui,, LI Qingbiao,, ZHANG Shuwen, HUANG Youlin   

  1. (1. College of Food and Bioengineering, Jimei University, Xiamen 361021, China;2. Fujian Provincial Key Laboratory of Food Microbiology and Engineering, Xiamen 361021, China;3. Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China; 4. DAMIN Foodstuff (Zhangzhou) Co. Ltd., Xiamen 361021, China; 5. College of Science, Jimei University, Xiamen 361021, China)
  • Online:2020-10-25 Published:2020-10-23

摘要: 为探究乳酸菌发酵、酵母菌发酵及醋酸菌发酵对茶叶水溶液风味的影响,通过感官评价和顶空固相微萃取结合气相色谱-质谱联用法,分析3 种发酵红茶水溶液的挥发性成分。结果表明:酵母菌、醋酸菌、乳酸菌使红茶水溶液的果香显著增加,而花香、甜香和青草香降低;此外,酵母菌、乳酸菌还显著降低红茶水溶液烘烤香,而醋酸菌显著增加红茶水溶液烘烤香。酵母菌发酵红茶水溶液鉴定出46 种挥发性成分,与原红茶水溶液相比,增加了醇类物质(1 605.93 μg/L)、酯类物质(7 715.82 μg/L)和酸类物质(596.15 μg/L),降低了醛类物质(69.48 μg/L);醋酸菌发酵红茶水溶液鉴定出33 种挥发性成分,与原红茶水溶液相比,增加了醇类物质(178.33 μg/L)和酮类物质(14.15 μg/L),降低了烯烃类物质(19.37 μg/L);乳酸菌发酵红茶水溶液鉴定出32 种挥发性成分,与原红茶水溶液相比,增加了醇类物质(90.70 μg/L)、酯类物质(53.91 μg/L)和酮类物质(19.82 μg/L),降低了烯烃类物质(10.89 μg/L)。本研究发现乳酸菌发酵可显著提高红茶水溶液的香味可接受程度,其主要原因是醇类物质、酯类物质,酮类物质等成分增加引起果香香气强度增加。该研究结果为运用发酵技术提高红茶产品的风味提供了理论参考。

关键词: 红茶水溶液;发酵;挥发性成分;气相色谱-质谱法;感官评价

Abstract: The purpose of this work was to explore the effect of fermentation with lactic acid bacteria, yeast or acetic acid bacteria on the flavor of black tea infusion. The volatile components of fermented tea infusion were analyzed by sensory evaluation and headspace solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS). It was shown that fermentation remarkably increased the fruity aroma and decreased the flowery, sweet and grassy aroma of black tea infusion. Besides, the roasted aroma was reduced after fermentation with yeast or lactic acid bacteria, but was increased after acetic acid bacterial fermentation. A total of 46 volatile components were identified in the tea infusion fermented by yeast, in which the contents of alcohols, esters and acids increase to 1 605.93, 7 715.82 and 596.15 μg/L, respectively, whereas the content of aldehydes decreased (69.48 μg/L) compared with those before fermentation. A total of volatile components were identified in the tea infusion fermented by acetic acid bacteria, in which the contents of alcohols and ketones increased respectively to 178.33 and 14.15 μg/L, while the content of olefins decreased to 19.37 μg/L relative to those before fermentation. A total of 32 volatile components were identified in the tea infusion fermented by lactic acid bacteria, in which the contents of alcohols, esters and ketones increased respectively to 90.70, 53.91 and 19.82 μg/L, while the content of olefins decreased to 10.89 μg/L relative to those before fermentation. This study showed that lactic acid bacterial fermentation could improve the aroma acceptability of black tea infusion, mainly because the intensity of fruity aroma was enhanced with increasing amounts of alcohols, esters and ketones. The results of this study provide a theoretical rationale for applying fermentation to improve the flavor of black tea products.

Key words: black tea infusion; fermentation; volatile components; gas chromatography-mass spectrometry; sensory evaluation

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