蜂蜜多酚提取物对大鼠急性酒精性肝损伤的保护作用

doi:10.7506/spkx1002-6630-20191113-170

食品科学 ›› 2020, Vol. 41 ›› Issue (21): 154-159.doi: 10.7506/spkx1002-6630-20191113-170

蜂蜜多酚提取物对大鼠急性酒精性肝损伤的保护作用

黄颖，谭书明，陈萍，陈小敏，宋长军

（1.贵州大学酿酒与食品工程学院，贵州贵阳 550025；2.贵州省农畜产品贮藏与加工重点实验室，贵州贵阳 550025）

发布日期:2020-11-23
基金资助:
黔科合重大专项（[2013]6006-2）

Protective Effect of Polyphenols Extracted from Honey against Acute Alcoholic Liver Injury in Rats

HUANG Ying, TAN Shuming, CHEN Ping, CHEN Xiaomin, SONG Zhangjun

1. School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China; 2. Guizhou Key Laboratory for Storage and Processing of Agricultural and Animal Products, Guiyang 550025, China

Published:2020-11-23

摘要/Abstract

摘要： 目的：研究蜂蜜多酚提取物对大鼠急性酒精性肝损伤的保护作用。方法：大鼠被随机分为空白对照组、模型组、药物对照组（100 mg/（kg mb·d））以及蜂蜜多酚提取物低、高剂量组（100、200 mg/（kg mb·d）），连续灌胃30 d后，除空白对照组外，其余各组给予体积分数56%的白酒（15 mL/kg mb）建立急性肝损伤模型。12 h后测定血清转氨酶活力、肝脏抗氧化和抗炎指标；实时荧光定量聚合酶链式反应法检测细胞外信号调节蛋白激酶（extracellular signal-regulated kinase，ERK）、c-Jun N端激酶（c-Jun N-terminal kinase，JNK）及p38激酶（p38 kinase，p38）mRNA表达量；苏木精-伊红染色观察肝组织病理学变化。结果：与模型组相比，各剂量组均能降低谷丙转氨酶、谷草转氨酶、丙二醛、肿瘤坏死因子-α水平，提高肝脏超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活力及还原型谷胱甘肽水平，下调ERK、JNK、p38 mRNA表达量，并改善肝脏病理学变化情况。结论：蜂蜜多酚提取物对大鼠急性酒精性肝损伤具有明显的保护作用，其机制可能与其抗氧化和抗炎活性有关。

关键词: 蜂蜜多酚提取物；急性酒精性肝损伤；保护作用；抗氧化活性；抗炎活性

Abstract: Purpose: To investigate the protective effect of honey polyphenol extract (HPE) on acute alcoholic hepatic injury in rats. Methods: Rats were randomly divided into blank control, model control, drug control (100 mg/(kg mb·d)), low- and high-dose HPE groups (100 and 200 mg/(kg mb·d)). After intragastric administration for 30 consecutive days, all rats except those in the blank control group were administered with Chinese Baiju containing 56% (V/V) alcohol at a dose of 15 mL/kg mb by gavage to establish an acute liver injury model. Twelve hours later, serum transaminase activity and antioxidant and anti-inflammatory indexes in liver were measured. The relative mRNA expression levels of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 kinase (p38) were determined by real-time quantitative polymerase chain reaction (qPCR). Hematoxylin-eosin (HE) staining was performed to observe the pathological changes of liver tissues. Results: Compared with the model group, the levels of alanine aminotransferase (ALT), aspartate aminotransaminase (AST), malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) were decreased in the HPE treatment groups, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and the level of reduced glutathione (GSH) were increased. Moreover, HPE downregulated the relative mRNA expression of ERK, JNK and p38, and improved the pathological changes of liver tissues. Conclusion: HPE has a protective effect on acute alcoholic liver injury in rats, and the underlying mechanism may be related to its antioxidant and anti-inflammatory activities.

Key words: honey polyphenol extract; acute alcoholic liver injury; protective effect; antioxidant activity; anti-inflammatory activity

中图分类号:

S896.6

黄颖，谭书明，陈萍，陈小敏，宋长军. 蜂蜜多酚提取物对大鼠急性酒精性肝损伤的保护作用[J]. 食品科学, 2020, 41(21): 154-159.

HUANG Ying, TAN Shuming, CHEN Ping, CHEN Xiaomin, SONG Zhangjun. Protective Effect of Polyphenols Extracted from Honey against Acute Alcoholic Liver Injury in Rats[J]. FOOD SCIENCE, 2020, 41(21): 154-159.

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蜂蜜多酚提取物对大鼠急性酒精性肝损伤的保护作用

Protective Effect of Polyphenols Extracted from Honey against Acute Alcoholic Liver Injury in Rats

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