核桃低聚肽对大鼠胃黏膜损伤的保护作用

doi:10.7506/spkx1002-6630-20200329-426

食品科学 ›› 2021, Vol. 42 ›› Issue (7): 191-197.doi: 10.7506/spkx1002-6630-20200329-426

核桃低聚肽对大鼠胃黏膜损伤的保护作用

刘睿，珠娜，郝云涛，刘欣然，康家伟，毛瑞雪，胡佳妮，于晓晨，李臻，李勇

（北京大学医学部公共卫生学院，北京 100191）

出版日期:2021-04-15 发布日期:2021-05-17

Protective Effect of Walnut Oligopeptides against Gastric Mucosal Injury of Rats

LIU Rui, ZHU Na, HAO Yuntao, LIU Xinran, KANG Jiawei, MAO Ruixue, HU Jiani, YU Xiaochen, LI Zhen, LI Yong

(School of Public Health, Health Science Center, Peking University, Beijing 100191, China)

Online:2021-04-15 Published:2021-05-17

摘要/Abstract

摘要： 本实验通过建立无水乙醇诱导的大鼠胃黏膜损伤模型，探讨核桃低聚肽（walnut oligopeptides，WOPs）对胃黏膜损伤的保护作用及可能机制。雄性SD大鼠根据体质量被随机分为8 组（n＝10）：正常对照组、模型对照组、乳清蛋白组（440 mg/kg mb）、奥美拉唑组（20 mg/kg mb）、3 个WOPs剂量组（220、440、880 mg/kg mb）及1 个WOPs与牛骨胶原低聚肽（bovine collagen oligopeptides，BCOPs）的配伍组（WOPs 440 mg/kg mb＋BCOPs 1.5 g/kg mb）。WOPs灌胃干预30 d后，采用无水乙醇5 mL/kg mb灌胃造模，随后进行胃黏膜损伤观察及评分，并测定血清丙氨酸氨基转移酶（alanine aminotransferase，ALT）和天冬氨酸氨基转移酶（aspartate aminotransferase，AST）水平及胃组织胃蛋白酶原（pepsinogen，PG）、黏蛋白（mucin，MUC）、胃泌素（gastrin，GAS）质量浓度。结果发现，WOPs干预明显减轻乙醇所致的胃黏膜出血、水肿及糜烂，抑制血清ALT、AST水平的上调和胃组织PGI、PGII、GAS质量浓度的升高，促进MUC的合成。研究结果进一步说明WOPs对无水乙醇所诱导的大鼠胃黏膜损伤具有较好的保护作用，其主要作用机制与减轻乙醇对胃黏膜的直接损伤，抑制PG、GAS的合成分泌，促进MUC的合成有关。

关键词: 核桃低聚肽；无水乙醇；胃黏膜损伤

Abstract: The study investigated the protective effect of walnut oligopeptides (WOPs) against absolute ethanol-induced gastric injury, and it also explored the possible underlying mechanism. Male Sprague Dawley (SD) rats were randomly divided into eight groups based on body mas (n = 10), including normal, model, whey protein (440 mg/kg mb), omeprazole (20 mg/kg mb), low-, medium- and high-dose WOP (220, 440, 880 mg/kg mb, respectively) and WOPs (440 mg/kg mb) + bovine collagen oligopeptides (BCOPs, 1.5 g/kg mb) groups. After oral gavage for 30 days, the rats were administered with 5 mL/kg mb of absolute ethanol to induce gastric ulcer, then the gross lesion of gastric tissue was observed and scored, and the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gastric mucin (MUC), pepsinogen (PG) and gastrin (GAS) were detected. The results showed that administration with WOPs markedly mitigated the hemorrhagic gastric lesions caused by ethanol in rats, inhibited the increase in serum ALT and AST levels and gastric PGI, PGII and GAS levels, and enhanced MUC biosynthesis. These results indicated that WOPs have obvious protective effects against ethanol-induced gastric mucosal injury in rats mainly by attenuating the gastric mucosal damage directly caused by ethanol, inhibiting the biosynthesis of PG and GAS, and enhancing MUC biosynthesis.

Key words: walnut oligopeptides; absolute ethanol; gastric mucosal injury

中图分类号:

R151.3

刘睿，珠娜，郝云涛，刘欣然，康家伟，毛瑞雪，胡佳妮，于晓晨，李臻，李勇. 核桃低聚肽对大鼠胃黏膜损伤的保护作用[J]. 食品科学, 2021, 42(7): 191-197.

LIU Rui, ZHU Na, HAO Yuntao, LIU Xinran, KANG Jiawei, MAO Ruixue, HU Jiani, YU Xiaochen, LI Zhen, LI Yong. Protective Effect of Walnut Oligopeptides against Gastric Mucosal Injury of Rats[J]. FOOD SCIENCE, 2021, 42(7): 191-197.

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核桃低聚肽对大鼠胃黏膜损伤的保护作用

Protective Effect of Walnut Oligopeptides against Gastric Mucosal Injury of Rats

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