食品科学 ›› 2018, Vol. 39 ›› Issue (23): 155-160.doi: 10.7506/spkx1002-6630-201823024

• 营养卫生 • 上一篇    下一篇

准噶尔山楂残渣多糖对D-半乳糖衰老模型小鼠的影响

马 雪1,吕海英1,2,*,李 进1,2,张 玉3,张 俊3   

  1. 1.新疆特殊环境物种保护与调控生物学实验室,新疆 乌鲁木齐 830054;2.新疆师范大学生命科学学院,新疆 乌鲁木齐 830054;3.干旱区植物逆境生物学重点实验室,新疆 乌鲁木齐 830054
  • 出版日期:2018-12-15 发布日期:2018-12-17
  • 基金资助:
    新疆自治区重点实验室项目(XJDX1414-2015-03);国家自然科学基金青年科学基金项目(31200300);国家自然科学基金地区科学基金项目(31560058);自治区教育厅普通本科高等学校重点实验室项目(xjtswz-2016-08)

Effects of Polysaccharides from Crataegus songorica K. Koch. Fruits on D-Galactose-Induced Aging Mice

MA Xue1, Lü Haiying1,2,*, LI Jin1,2, ZHANG Yu3, ZHANG Jun3   

  1. 1. Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, ürümqi 830054, China; 2. College of Life Science, Xinjiang Normal University, ürümqi 830054, China; 3. Key Laboratory of Plant Stress Biology in Arid Land, ürümqi 830054, China
  • Online:2018-12-15 Published:2018-12-17

摘要: 研究准噶尔山楂残渣中的多糖对D-半乳糖致衰老小鼠的影响。采用水提醇沉法从准噶尔山楂残渣中提取多糖,对D-半乳糖致衰老小鼠灌胃不同剂量(200、400、800 mg/kg)的多糖,测定小鼠肝、脑组织及血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、总抗氧化能力(total antioxidative capability,T-AOC)、丙二醛(malondialdehyde,MDA)含量,并观察肝、脑组织病理形态学变化。结果表明:与模型组相比,多糖高剂量组小鼠肝组织和血清中GSH-Px活力显著升高(P<0.05,P<0.01);脑组织和血清中T-AOC显著升高(P<0.01,P<0.05);肝、脑组织和血清中MDA含量显著降低(P<0.05,P<0.01)。多糖中、高剂量组小鼠肝索结构完整,肝细胞内无明显脂肪空泡;海马区神经元结构较为清晰,细胞排列整齐。因此准噶尔山楂多糖对D-半乳糖引起的小鼠衰老有一定抑制作用,这为新疆特有植物准噶尔山楂的高效利用提供了依据。

关键词: 准噶尔山楂, 多糖, D-半乳糖, 衰老

Abstract: This purpose of this study was to explore the effect of polysaccharides extracted from the residue left after the extraction of anthocyanins from Crataegus songorica K. Koch. fruits on D-galactose-induced aging mice. The polysaccharides were prepared by water extraction and alcohol precipitation and were orally administered to D-galactoseinduced aging mice at three different doses (200, 400 and 800 mg/kg). Glutathione peroxidase (GSH-Px) activity, total antioxidative capability (T-AOC) and malondialdehyde (MDA) content in brain, liver and serum were measured. Histological extraction of liver and brain tissues was carried out as well. The results showed that high-dose polysaccharide could significantly improve the activity of GSH-Px in liver and serum (P < 0.05, P < 0.01), increase T-AOC in brain and liver (P < 0.01, P < 0.05), decrease the content of MDA in liver, brain and serum (P < 0.05, P < 0.01) when compared with the model group. The structure of liver lobule was intact in mice from the medium- and high-dose polysaccharide group, with no apparent fat vacuoles being observed in hepatocytes. The structure of hippocampal nerve cells, arranged orderly, was clear. In conclusion, the polysaccharides from Crataegus songorica K. Koch. fruits can protect against D-galactose-induced aging in mice, which provides a clue for the efficient utilization of Crataegus songorica K. Koch..

Key words: Crataegus songorica K. Koch., polysaccharides, D-galactose, aging

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