食品科学 ›› 2021, Vol. 42 ›› Issue (13): 136-142.doi: 10.7506/spkx1002-6630-20200715-210

• 营养卫生 • 上一篇    下一篇

乳铁蛋白对炎症性肠炎大鼠肠道微生态的正向调节作用

赵晓,徐境含,崔东影,徐珒昭,刘洋,滕国新,许晓曦   

  1. (1.东北农业大学食品学院,黑龙江 哈尔滨 150030;2.武警工程大学装备管理与保障学院,陕西 西安 710086;3.黑龙江飞鹤乳业有限公司,北京 100015;4.内蒙古蒙牛乳业(集团)股份有限公司,内蒙古 呼和浩特 011500)
  • 出版日期:2021-07-15 发布日期:2021-07-27
  • 基金资助:
    内蒙古自治区科技成果转化项目(CGZH2018035)

Positive Regulation of Lactoferrin on Gut Micro-Ecology of Rats with Inflammatory Bowel Disease

ZHAO Xiao, XU Jinghan, CUI Dongying, XU Jinzhao, LIU Yang, TENG Guoxin, XU Xiaoxi   

  1. (1. School of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. Institute Equipment Management and Support, Engineering University of Peoples Armed Police Force, Xi’an 710086, China; 3. Heilongjiang Feihe Dairy Co., Ltd., Beijing 100015, China; 4. Inner Mongolia Mengniu Dairy Industry (Group) Co., Ltd., Hohhot 011500, China)
  • Online:2021-07-15 Published:2021-07-27

摘要: 本研究采用葡聚糖硫酸钠(dextran sulphate sodium,DSS)刺激以构建SD大鼠炎症性肠炎(inflammatory bowel disease,IBD)模型,通过高通量测序分析乳铁蛋白(lactoferrin,LF)对大鼠结肠粪便肠道菌群α-多样性、物种差异性分布、KEGG信号通路相关操作分类单元(operational taxonomic unit,OTU)丰度的影响。结果表明,LF有助于缓解DSS刺激所引起的α-多样性降低和优势菌群——厚壁菌门(Firmicutes)与拟杆菌门(Bacteroidetes)的比例失调;与DSS组相比,高剂量LF提高了患IBD大鼠乳杆菌科(Lactobacillaceae)(12% vs. 40%)、瘤胃菌科(Ruminococcaceae)(17% vs. 27%)等菌群的相对丰度,降低了拟杆菌科(Bacteroides)(28% vs. 8%)、普雷沃氏菌科(Prevotellaceae)(13% vs. 6%)等菌群的相对丰度。KEGG信号通路OTU丰度分析表明,LF的摄入降低了患IBD大鼠菌群中与癌症、感染性疾病等人类疾病、免疫系统和细胞及遗传相关信号通路的OTU丰度,并降低了这些疾病的发生风险。综上,LF的摄入对患IBD大鼠结肠微生态具有正向调节作用,可使其肠道菌群的组成更接近于健康大鼠,且有降低其他伴随性疾病风险的可能。

关键词: 乳铁蛋白;肠道菌群;炎症性肠炎;SD大鼠;KEGG信号通路

Abstract: The effect of lactoferrin (LF) on the α-diversity and composition, as well as the abundance of KEGG signaling pathway-related operational taxonomic unit (OTU) in the colonic microbiota of rats with inflammatory bowel disease (IBD) induced by dextran sulphate sodium (DSS) was analyzed by high-throughput sequencing. The results showed that LF alleviated the reduction of α-diversity and the imbalance between the dominant phyla Firmicutes and Bacteroidetes caused by DSS stimulation. LF increased the abundance of Lactobacillaceae (12% vs. 40%) and Ruminococcaceae (17% vs. 27%), and decreased Bacteroides (28% vs. 8%) and Prevotellaceae (13% vs. 6%). LF administration reduced the abundance of OTUs associated with human diseases such as cancer and infectious diseases, immune system, cells and genetics-related signaling pathways in the gut microbiota in IBD rats. The results of this study indicated that LF can play a beneficial role in regulating the colonic micro-ecology of IBD rats and modulating the microbiota composition to be similar to that of healthy rats, thereby having the potential to reduce the risk of other concomitant diseases.

Key words: lactoferrin; gut microbiota; inflammatory bowel disease; SD rats; KEGG signaling pathway

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