竹叶提取物和敲除转录因子DAL80对酿酒酵母精氨酸代谢的调控互作影响

doi:10.7506/spkx1002-6630-20210201-022

食品科学 ›› 2021, Vol. 42 ›› Issue (16): 76-82.doi: 10.7506/spkx1002-6630-20210201-022

竹叶提取物和敲除转录因子DAL80对酿酒酵母精氨酸代谢的调控互作影响

刘小杰，娄行行，陈启和

（1.上海城建职业学院，上海 201415；2.浙江大学生物系统工程与食品科学学院，浙江杭州 310058）

发布日期:2021-08-27
基金资助:
国家自然科学基金面上项目（31871904；31171734）

Interactive Effects of Addition of Bamboo Leaf Extract and Knockout of Transcriptional Factor DAL80 on Arginine Metabolism in Saccharomyces cerevisiae

LIU Xiaojie, LOU Hanghang, CHEN Qihe

(1. Shanghai Urban Construction Vocational College, Shanghai 201415, China; 2. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China)

Published:2021-08-27

摘要/Abstract

摘要： 以竹叶提取物为外源添加物，采用酿酒酵母DAL80敲除菌（DAL80Δ）研究添加天然产物和基因工程手段对精氨酸（arginine，Arg）代谢调控的影响。实验发现，DAL80敲除和添加竹叶提取物均能影响Arg代谢，且作用机制有所不同。敲除DAL80可提高尿素代谢相关基因（DUR1,2、DUR3、DAL82）的转录水平，以加快酿酒酵母对氨基甲酸乙酯前体物质的消耗从而抑制其形成；竹叶提取物主要通过降低酿酒酵母细胞Arg的利用率以减少尿素的合成，以此减少尿素转化为氨基甲酸乙酯。当两者同时作用时，竹叶提取物虽会削弱DAL80敲除带来的抑制影响，但DAL80敲除对酿酒酵母细胞氮代谢的影响仍占主导地位。

关键词: 黄酒；精氨酸；尿素；转录因子DAL80；竹叶提取物

Abstract: In this study, the effects of addition of bamboo leaf extract (BLE) and knockout of the transcriptional factor DAL80 gene on the regulation of arginine (Arg) metabolism were investigated. It was found that both DAL80 knockout and BLE addition affected arginine metabolism, but by different mechanisms. DAL80 knockout could improve the transcriptional level of urea metabolism-related genes (DUR1,2, DUR3 and DAL82), accelerating the consumption of ethyl carbamate precursors by Saccharomyces cerevisiae to inhibit the formation of ethyl carbamate. BLE reduced the utilization of Arg by S. cerevisiae and consequently the synthesis of urea, finally reducing the formation of ethyl carbamate. When working simultaneously, BLE could weaken the inhibitory effect of DAL80 knockout, but the effect of DAL80 knockout on nitrogen metabolism in S. cerevisiae was still dominant.

Key words: Chinese yellow rice wine; arginine; urea; transcriptional factor DAL80; bamboo leaf extract

中图分类号:

TS261.11

刘小杰，娄行行，陈启和. 竹叶提取物和敲除转录因子DAL80对酿酒酵母精氨酸代谢的调控互作影响[J]. 食品科学, 2021, 42(16): 76-82.

LIU Xiaojie, LOU Hanghang, CHEN Qihe. Interactive Effects of Addition of Bamboo Leaf Extract and Knockout of Transcriptional Factor DAL80 on Arginine Metabolism in Saccharomyces cerevisiae[J]. FOOD SCIENCE, 2021, 42(16): 76-82.

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竹叶提取物和敲除转录因子DAL80对酿酒酵母精氨酸代谢的调控互作影响

Interactive Effects of Addition of Bamboo Leaf Extract and Knockout of Transcriptional Factor DAL80 on Arginine Metabolism in Saccharomyces cerevisiae

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